About: Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recent research has been focused on the development of innovative magnetic micrometer-sized particles for specific applications in DNA analysis in food matrices and other real samples.Carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres were used for non-selective isolation of whole DNA from the sample. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. The magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation. Isolation of target DNA was verified by PCR using genus-specific primers R16-1 and LblMA-rev (Dubernet et al., 2002) in food supplements. Recent research has been focused on the development of innovative magnetic micrometer-sized particles for specific applications in DNA analysis in food matrices and other real samples.Carboxyl group-containing magnetic nonporous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres were used for non-selective isolation of whole DNA from the sample. This procedure was used for PCR-ready DNA isolation and identification of Lactobacillus, Bifidobacterium and psychrotrophic bacteria in different dairy products or sediments. The magnetic microparticles P(HEMA-co-GMA) functionalised with streptavidin were used for immobilisation of biotinylated DNA probe and for selective isolation of target DNA from complex samples (e.g. food supplements) using DNA/DNA hybridisation. Isolation of target DNA was verified by PCR using genus-specific primers R16-1 and LblMA-rev (Dubernet et al., 2002) in food supplements. (en)
Title	Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles (en)
skos:prefLabel	Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles Non-selective and selective isolation of DNA from food matrices and other real samples by magnetic particles (en)
skos:notation	RIV/00216305:26310/09:PU87650!RIV11-MSM-26310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B08070)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Tvrdíková, Jana Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	329746
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/09:PU87650
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	magnetic particles, streptavidin, DNA, PCR (en)
http://linked.open.../riv/klicoveSlovo	DNA PCR streptavidin magnetic particles
http://linked.open...ontrolniKodProRIV	[F932C04748CB]
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Rittich, Bohuslav Španová, Alena Tvrdíková, Jana
http://localhost/t...ganizacniJednotka	26310

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