About: Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution

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About: Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Low-molecular mass proteins rich in cysteines called metallothioneins (MT) can be considered as markers for the pollution of the environment by metals. Here, we report on suggestion for an automated procedure for the isolation of MT followed by voltammetric analysis. Primarily, we optimized the automated detection of MTusing an electrochemical analyser. It was found that the most sensitive and repeatable analyses are obtained at a temperature of 4C for the supporting electrolyte. Further, we optimized experimental conditions for the isolation of MT by using antibody-linked paramagnetic microparticles. Under the optimal conditions (4 h long interaction between the microparticles and MT), the microparticles were tested on isolation of various amounts of MT. The lowest isolated amount of MT by antibody-linked paramagnetic microparticles was 5 mg ml of MT (50 ng). The automated procedure of MT isolation was further tested on isolation of MT from guppy fish (Poecilia reticulata) treated with silver(I) ions Low-molecular mass proteins rich in cysteines called metallothioneins (MT) can be considered as markers for the pollution of the environment by metals. Here, we report on suggestion for an automated procedure for the isolation of MT followed by voltammetric analysis. Primarily, we optimized the automated detection of MTusing an electrochemical analyser. It was found that the most sensitive and repeatable analyses are obtained at a temperature of 4C for the supporting electrolyte. Further, we optimized experimental conditions for the isolation of MT by using antibody-linked paramagnetic microparticles. Under the optimal conditions (4 h long interaction between the microparticles and MT), the microparticles were tested on isolation of various amounts of MT. The lowest isolated amount of MT by antibody-linked paramagnetic microparticles was 5 mg ml of MT (50 ng). The automated procedure of MT isolation was further tested on isolation of MT from guppy fish (Poecilia reticulata) treated with silver(I) ions (en)
Title	Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution (en)
skos:prefLabel	Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution (en)
skos:notation	RIV/00216305:26220/11:PU94013!RIV15-MSM-26220___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA102/08/1546), P(KAN208130801), S, Z(MSM0021622412), Z(MSM0021630513), Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Škutková, Helena Provazník, Ivo Adam, Vojtěch Hubálek, Jaromír Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	219525
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/11:PU94013
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antibody, microparticles, voltammetry, metallothionen, pollution (en)
http://linked.open.../riv/klicoveSlovo	microparticles metallothionen pollution antibody voltammetry
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[963303298918]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF ENVIRONMENTAL MONITORING
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	New design and exploiting nanobiosensors and nanosensors to target medicine (NANOSEMED) Miniaturized intelligent systems and nanostructured elektrodes for chemical, biological, and pharmaceutical applications (NANIMEL)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	2011
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Hubálek, Jaromír Húska, Dalibor Kizek, René Provazník, Ivo Trnková, Libuše Škutková, Helena
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Advanced Electronic Communication Systems and Technologies (ELCOM) Veterinary aspects of food safety and quality
issn	1464-0325
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	26220

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