About: Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO5. Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO5. Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data (en)
Title	Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages (en)
skos:prefLabel	Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages (en)
skos:notation	RIV/00216305:26220/10:PU89374!RIV11-GA0-26220___
http://linked.open...avai/riv/aktivita	P S V Z
http://linked.open...avai/riv/aktivity	P(GD102/09/H083), S, V, Z(MSM0021630513)
http://linked.open...iv/cisloPeriodika	15
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Ryvolová, Markéta Provazník, Ivo Adam, Vojtěch Hubálek, Jaromír Kizek, René Sochor, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	259920
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/10:PU89374
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antioxidant activity, spectrometry, trolox, gallic acid (en)
http://linked.open.../riv/klicoveSlovo	antioxidant activity spectrometry gallic acid trolox
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[303295AAB9EE]
http://linked.open...i/riv/nazevZdroje	MOLECULES
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Information Technology in Biomedical Engineering
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	2010
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Havel, Ladislav Hubálek, Jaromír Kizek, René Provazník, Ivo Ryvolová, Markéta Salaš, Petr Sochor, Jiří Trnková, Libuše Zehnálek, Josef Kryštofová, Jana
http://linked.open...n/vavai/riv/zamer	Advanced Electronic Communication Systems and Technologies (ELCOM)
issn	1420-3049
number of pages	23 (xsd:int)
http://localhost/t...ganizacniJednotka	26220

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