About: Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection

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About: Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, %22current R%22: 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled In this paper, we report on an investigation of affecting guppy fishes (Poecilia reticulata) by silver ions (0, 0.3, 0.6, 1.2, 2.5, and 5 mu M) for seven days under well-controlled experimental conditions. To observe the physiological changes, we attempted to determine metallothionein (MT) as a biomarker of heavy metal stress. For this purpose, we proposed a sensor utilizing a carbon electrode coupled with flow injection analysis. The experimental conditions, which have been optimized, were as follows-applied potential: 750 mV, mobile phase: Britton-Robinson buffer (pH 1.9) with How rate of 0.6 ml/min, time filter: 2.5 s, %22current R%22: 1 mu A. Under these conditions, the detection limit of MT was estimated as 100 pM. After the optimizing step, the fish tissues were measured. Based on the results obtained, MT content increased with increasing dose of silver ions and time of the treatment. The results obtained were in good correlation with those obtained by adsorptive transfer stripping technique coupled (en)
Title	Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection (en)
skos:prefLabel	Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection Bio-Assessing of Environmental Pollution via Monitoring of Metallothionein Level Using Electrochemical Detection (en)
skos:notation	RIV/00216305:26220/08:PU78016!RIV13-MSM-26220___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA526/07/0674), Z(MSM0021622412)
http://linked.open...iv/cisloPeriodika	9-10
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Hubálek, Jaromír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	357993
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/08:PU78016
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Amperometric detection, biomarker, electrochemical sensors, fish, flow injection analysis, heavy metal, metallothionein, poecilia reticulata, silver (en)
http://linked.open.../riv/klicoveSlovo	flow injection analysis electrochemical sensors fish heavy metal Amperometric detection silver metallothionein biomarker poecilia reticulata
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[21DD34E00C20]
http://linked.open...i/riv/nazevZdroje	IEEE SENSORS JOURNAL
http://linked.open...in/vavai/riv/obor	JB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Ultrasensitive analysis of silver in waters
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Hubálek, Jaromír Kizek, René Křížková, Soňa Stejskal, Karel Trnková, Libuše Zehnálek, Josef
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
issn	1530-437X
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	26220

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