About: Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recording of monophasic action potentials (MAPs) by optical method using voltage sensitive dyes has been established as possibility to measure membrane potential changes in certain models. We studied two procedures of isolated rabbit hearts loading with di-4-ANEPPS - %22slow%22 method (perfusion with low concentration of dye for longer time) or %22fast%22 method (single microinjection of highly concentrated bolus of the dye in the aorta). Signal-to-noise ratio was evaluated in each minute of continuous exposure to excitatory light and persistence of dye in the tissue was estimated. We conclude that both methods enable us to record MAPs, but during long exposure to light the persistence of the dye in the tissue is insufficient in %22fast%22 method. Although the %22slow%22 loading affects electrophysiological properties of the heart, in case of rabbit heart it is negligible since its relative resistance to the side-effects of di-4-ANEPPS. Recording of monophasic action potentials (MAPs) by optical method using voltage sensitive dyes has been established as possibility to measure membrane potential changes in certain models. We studied two procedures of isolated rabbit hearts loading with di-4-ANEPPS - %22slow%22 method (perfusion with low concentration of dye for longer time) or %22fast%22 method (single microinjection of highly concentrated bolus of the dye in the aorta). Signal-to-noise ratio was evaluated in each minute of continuous exposure to excitatory light and persistence of dye in the tissue was estimated. We conclude that both methods enable us to record MAPs, but during long exposure to light the persistence of the dye in the tissue is insufficient in %22fast%22 method. Although the %22slow%22 loading affects electrophysiological properties of the heart, in case of rabbit heart it is negligible since its relative resistance to the side-effects of di-4-ANEPPS. (en)
Title	Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart (en)
skos:prefLabel	Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart Comparison of Two Procedures of Loading with Voltage-Sensitive Dye Di-4-ANEPPS in Rabbit Isolated Heart (en)
skos:notation	RIV/00216305:26220/08:PU75738!RIV10-MSM-26220___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA102/07/1473), P(GP102/07/P521), Z(MSM0021630513)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kolářová, Jana Provazník, Ivo
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	360708
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/08:PU75738
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Recording, Voltage-Sensitive Dye, Action Potentials (en)
http://linked.open.../riv/klicoveSlovo	Recording Voltage-Sensitive Dye Action Potentials
http://linked.open...ontrolniKodProRIV	[F8B000B7C863]
http://linked.open...v/mistoKonaniAkce	Bologna
http://linked.open...i/riv/mistoVydani	Bologna, Italy
http://linked.open...i/riv/nazevZdroje	Computers in Cardiology
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization Methods of analysis of mechanical and electrical activity of the heart in experimental cardiology
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Kolářová, Jana Provazník, Ivo Nováková, Marie Nogová, Kateřina
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2008-09-14 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Advanced Electronic Communication Systems and Technologies (ELCOM)
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	IEEE
http://localhost/t...ganizacniJednotka	26220

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software