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| rdf:type
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| Description
| - Voltage-sensitive dyes (VSD's) are used in basic cardiology research to record transient potential changes in various cardiac preparations. In our laboratory, action potentials (APs) are recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining with this VSD were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff. Staining was done either by coronary perfusion with low dye concentration or with bolus injection of highly concentrated dye into the aorta. Electrogram and coronary flow were followed continuously. During the staining and washout from dye, prominent electrophysiological changes occurred such as decrease of spontaneous heart rate, partial AV block and changes of ST-T segment, accompanied with decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded putative ischemic damage of myocardium. Good viability of stained preparation was proved by electron microscopy. Since in r
- Voltage-sensitive dyes (VSD's) are used in basic cardiology research to record transient potential changes in various cardiac preparations. In our laboratory, action potentials (APs) are recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining with this VSD were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff. Staining was done either by coronary perfusion with low dye concentration or with bolus injection of highly concentrated dye into the aorta. Electrogram and coronary flow were followed continuously. During the staining and washout from dye, prominent electrophysiological changes occurred such as decrease of spontaneous heart rate, partial AV block and changes of ST-T segment, accompanied with decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded putative ischemic damage of myocardium. Good viability of stained preparation was proved by electron microscopy. Since in r (en)
- Voltage-sensitive dyes (VSD's) are used in basic cardiology research to record transient potential changes in various cardiac preparations. In our laboratory, action potentials (APs) are recorded by optical probe using di-4-ANEPPS. In this study, the effects of two different ways of staining with this VSD were compared in guinea pig and rabbit isolated hearts perfused according to Langendorff. Staining was done either by coronary perfusion with low dye concentration or with bolus injection of highly concentrated dye into the aorta. Electrogram and coronary flow were followed continuously. During the staining and washout from dye, prominent electrophysiological changes occurred such as decrease of spontaneous heart rate, partial AV block and changes of ST-T segment, accompanied with decrease in mean coronary flow. No production of hydroxyl radicals was found by HPLC which excluded putative ischemic damage of myocardium. Good viability of stained preparation was proved by electron microscopy. Since in r (cs)
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| Title
| - Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium
- Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium (en)
- Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium (cs)
|
| skos:prefLabel
| - Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium
- Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium (en)
- Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium (cs)
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| skos:notation
| - RIV/00216305:26220/08:PU71732!RIV10-GA0-26220___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA102/07/1473), P(GP102/07/P521), Z(MSM0021622402)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216305:26220/08:PU71732
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - di-4-ANEPPS, myocardium, rabbit, guinea pig (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - General Physiology and Biophysics
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kolářová, Jana
- Provazník, Ivo
- Táborská, Eva
- Nováková, Marie
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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