About: Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mass transport of the bulk of the analyte to the electrode and through the bioactive layer can be significantly improved by use of the nanoelectrode array and defined arrangement of protein film. This phenomenon has been studied by (i) atomic-force microscopy, (ii) electrochemical measurements of PSII activity, and (iii) digital simulations for an oriented monolayer of histidine-tagged photosystem II (PSII) immobilized on nitrilotriacetic acid (NTA)-modified gold electrodes. The output signal of the electrochemical biosensor is controlled by (i) mass transport from the bioactive layer to electrode and (ii) mass transport between the bulk of the analyte and the electrode. Mass transport through the bioactive layer was electrochemically studied for PSII self-assembled on gold screen-printed electrodes. A densely packed monolayer of PSII has a significant shielding effect toward the diffusion of redox mediator duroquinone (DQ). Mass transport to the planar electrode surface was improved by co-immobilizat Mass transport of the bulk of the analyte to the electrode and through the bioactive layer can be significantly improved by use of the nanoelectrode array and defined arrangement of protein film. This phenomenon has been studied by (i) atomic-force microscopy, (ii) electrochemical measurements of PSII activity, and (iii) digital simulations for an oriented monolayer of histidine-tagged photosystem II (PSII) immobilized on nitrilotriacetic acid (NTA)-modified gold electrodes. The output signal of the electrochemical biosensor is controlled by (i) mass transport from the bioactive layer to electrode and (ii) mass transport between the bulk of the analyte and the electrode. Mass transport through the bioactive layer was electrochemically studied for PSII self-assembled on gold screen-printed electrodes. A densely packed monolayer of PSII has a significant shielding effect toward the diffusion of redox mediator duroquinone (DQ). Mass transport to the planar electrode surface was improved by co-immobilizat (en)
Title	Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement (en)
skos:prefLabel	Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement (en)
skos:notation	RIV/00216305:26220/05:PU83110!RIV11-MPO-26220___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(FD-K2/53), P(FT-TA/089), P(GA522/03/0659), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Masojídek, Jiří Krejčí, Jan Jakubka, Luboš Maly, Jiří Šteffan, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta elektrotechniky a komunikačních technologií
http://linked.open...dnocenehoVysledku	531159
http://linked.open...ai/riv/idVysledku	RIV/00216305:26220/05:PU83110
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein monolayer, photosystem II, nanostructured electrodes, biosensors, mass transport (en)
http://linked.open.../riv/klicoveSlovo	mass transport nanostructured electrodes biosensors protein monolayer photosystem II
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[BB90E0D95AC1]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	IBIS - Inteligent biosensor system for environmental detection of pesticides and herbicides. Improvement of photosynthetic biosenzors and bioassays for routine herbicide residues detection and non-target organisms risk assessment The research of new methods of total toxicity analysis in food industry and the testing of new methods on pesticide toxicity analyser prototype.
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	381
http://linked.open...iv/tvurceVysledku	Masojídek, Jiří Šteffan, Pavel Krejčí, Jan Jakubka, Luboš Maly, Jiří
http://linked.open...ain/vavai/riv/wos	000228728200012
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	1618-2642
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	26220

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