| Attributes | Values |
|---|
| rdf:type
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| Description
| - Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that during the lo
- Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that during the lo (en)
- Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that during the lo (cs)
|
| Title
| - Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models
- Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models (en)
- Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models (cs)
|
| skos:prefLabel
| - Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models
- Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models (en)
- Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models (cs)
|
| skos:notation
| - RIV/00216305:26220/05:PU50087!RIV06-GA0-26220___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA102/04/0472), P(GA305/04/1385), Z(MSM0021630513)
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216305:26220/05:PU50087
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - tissue, voltage-sensitive dye, animal model (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...v/mistoKonaniAkce
| |
| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Provazník, Ivo
- Nováková, Marie
- Blaha, Milan
- Bardoňová, Jana
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| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| http://linked.open...n/vavai/riv/zamer
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| |
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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