About: Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM

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About: Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Computerised x-ray microtomography (microCT) is increasingly used in the 3D study of bone microarchitecture and in quantifying bone volume fractions. However, the volumetric resolution in labora- tory apparatus for small rodent studies is at best several microns linear and recognisable detail characterising forming, resting and resorbing surfaces is completely missing. Backscattered electron mode scanning electron microscopy (BSE SEM) of both macerated 3D samples and polished surfaces of blocks of PMMA embedded tissue provides this information but samples have to be cut and processed. The same PMMA material is good for confocal fluores- cence microscopy (CSLM) for both tissue morphology and the study of tetracycline and calcein mineralising front labels to 50– 200 microns deep to the block surface. With the recent acquisi- tion of SEM with variable chamber pressure to permit examina- tion of uncoated specimens, we are able to conduct CSLM after SEM for correlation studies. Here, we report new approa Computerised x-ray microtomography (microCT) is increasingly used in the 3D study of bone microarchitecture and in quantifying bone volume fractions. However, the volumetric resolution in labora- tory apparatus for small rodent studies is at best several microns linear and recognisable detail characterising forming, resting and resorbing surfaces is completely missing. Backscattered electron mode scanning electron microscopy (BSE SEM) of both macerated 3D samples and polished surfaces of blocks of PMMA embedded tissue provides this information but samples have to be cut and processed. The same PMMA material is good for confocal fluores- cence microscopy (CSLM) for both tissue morphology and the study of tetracycline and calcein mineralising front labels to 50– 200 microns deep to the block surface. With the recent acquisi- tion of SEM with variable chamber pressure to permit examina- tion of uncoated specimens, we are able to conduct CSLM after SEM for correlation studies. Here, we report new approa (en)
Title	Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM (en)
skos:prefLabel	Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM (en)
skos:notation	RIV/00216305:26210/12:PU99555!RIV13-MSM-26210___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kvasnica, Lukáš Zikmund, Tomáš
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta strojního inženýrství
http://linked.open...dnocenehoVysledku	128856
http://linked.open...ai/riv/idVysledku	RIV/00216305:26210/12:PU99555
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	x-ray microtomography,scanning electron microscopy,confocal fluorescence microscopy,rat femur (en)
http://linked.open.../riv/klicoveSlovo	rat femur confocal fluorescence microscopy x-ray microtomography scanning electron microscopy
http://linked.open...ontrolniKodProRIV	[ED72F99AC9C0]
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Zikmund, Tomáš Boyde, Alan Kvasnica, Lukáš
http://localhost/t...ganizacniJednotka	26210

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