About: System for coherence-controlled holographic microscopy of living cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Coherence Controlled Holographic Microscopy (CCHM) is a novel holographic technique for quantitative-phase contrast (QPC) biological observations particularly of living cells. Owing to the ordinary (low coherence) illumination source, the CCHM images are of low noise, deprived of coherence noise (speckles) and the lateral resolution is improved by a factor of 2 compared to classic holographic microscopes. Long-lasting time-lapse experiments require elimination of the CCHM optical system instability in order to achieve precise QPC measurement and to maintain correct CCHM adjustment for its low-coherence operation. The critical part of CCHM is the interferometer, which is very sensitive to temperature fluctuations and air turbulences. The temperature stabilization of the whole microscope without air turbulences is therefore required to provide stability for long-term observations of living cells. Novel heated microscope box and stage designed and constructed for this purpose are described in the paper. Coherence Controlled Holographic Microscopy (CCHM) is a novel holographic technique for quantitative-phase contrast (QPC) biological observations particularly of living cells. Owing to the ordinary (low coherence) illumination source, the CCHM images are of low noise, deprived of coherence noise (speckles) and the lateral resolution is improved by a factor of 2 compared to classic holographic microscopes. Long-lasting time-lapse experiments require elimination of the CCHM optical system instability in order to achieve precise QPC measurement and to maintain correct CCHM adjustment for its low-coherence operation. The critical part of CCHM is the interferometer, which is very sensitive to temperature fluctuations and air turbulences. The temperature stabilization of the whole microscope without air turbulences is therefore required to provide stability for long-term observations of living cells. Novel heated microscope box and stage designed and constructed for this purpose are described in the paper. (en)
Title	System for coherence-controlled holographic microscopy of living cells System for coherence-controlled holographic microscopy of living cells (en)
skos:prefLabel	System for coherence-controlled holographic microscopy of living cells System for coherence-controlled holographic microscopy of living cells (en)
skos:notation	RIV/00216305:26210/12:PU102376!RIV13-MPO-26210___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(FR-TI4/660), P(TE01020229)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Křížová, Aneta Veselý, Pavel Chmelík, Radim Antoš, Martin Čolláková, Jana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta strojního inženýrství
http://linked.open...dnocenehoVysledku	173034
http://linked.open...ai/riv/idVysledku	RIV/00216305:26210/12:PU102376
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Holographic microscopy, temperature control, culture chambers (en)
http://linked.open.../riv/klicoveSlovo	Holographic microscopy temperature control culture chambers
http://linked.open...ontrolniKodProRIV	[326C3F2F9177]
http://linked.open...v/mistoKonaniAkce	Ostravice
http://linked.open...i/riv/mistoVydani	Neuveden
http://linked.open...i/riv/nazevZdroje	Proceedings of SPIE
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of digital optics Central european institute of technology *Multimodal holographic microscope
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Chmelík, Radim Veselý, Pavel Antoš, Martin Čolláková, Jana Křížová, Aneta
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2012-09-03 (xsd:date)
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	978-80-244-3408-7
http://localhost/t...ganizacniJednotka	26210

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