| Attributes | Values |
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| rdf:type
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| Description
| - Nevertheless, it is important to investigate these effects in more details for better understanding of lipids properties and for accurate quantitation. Methods Sets of PCs with fixed fatty acyl (14:0, 17:0 or 18:1) in sn-1 position and varied in length and saturation degree of fatty acyls in sn-2 position of glycerol backbone were synthesized. Samples with various concentrations of lipids were mixed with several MALDI matrices including DHB, 9-aminoacridine and diaminonaphthalene. Results MALDI-MS investigation of synthesized PC sets revealed the dependence of the intensity of lipid-related peaks on fatty acyl lengths. The character of the dependence was changed depending on used matrices and concentrations of lipids. In case of DHB matrix, the dependence was not monotonic. Peak intensities increased with the chain length increasing for samples with higher lipids concentrations and decreased for lower concentrations. The fact of lipid peaks decrease for longer fatty acyl chains for samples containing lower lipids concentrations was common for different matrices, while the slope of this decrease was again not the same for various matrices. The weakest changes of relative peak intensities were observed for 9-aminoacridine matrix. The smallest shot-to-shot deviation of relative abundances was also obtained for 9-aminoacridine as a matrix. Conclusions The instrument response depends on fatty acyls composition of PCs. This dependence must be taken into account upon the quantitative MALDI-MS analysis of phospholipids. MALDI matrix must also be chosen with care to avoid distortion of estimation of lipid concentrations based on peak intensities. Authors acknowledge the support of projects No. LL1302 (ERC CZ) and No. CZ.1.07/2.3.00/30.0021 (MSMT CR). Novel Aspect Comprehensive study of PCs composition and concentration influence on the response of mass spectrometer with Orbitrap mass analyzer is provided. The effect of MALDI matrix on the response is considered as well.
- Nevertheless, it is important to investigate these effects in more details for better understanding of lipids properties and for accurate quantitation. Methods Sets of PCs with fixed fatty acyl (14:0, 17:0 or 18:1) in sn-1 position and varied in length and saturation degree of fatty acyls in sn-2 position of glycerol backbone were synthesized. Samples with various concentrations of lipids were mixed with several MALDI matrices including DHB, 9-aminoacridine and diaminonaphthalene. Results MALDI-MS investigation of synthesized PC sets revealed the dependence of the intensity of lipid-related peaks on fatty acyl lengths. The character of the dependence was changed depending on used matrices and concentrations of lipids. In case of DHB matrix, the dependence was not monotonic. Peak intensities increased with the chain length increasing for samples with higher lipids concentrations and decreased for lower concentrations. The fact of lipid peaks decrease for longer fatty acyl chains for samples containing lower lipids concentrations was common for different matrices, while the slope of this decrease was again not the same for various matrices. The weakest changes of relative peak intensities were observed for 9-aminoacridine matrix. The smallest shot-to-shot deviation of relative abundances was also obtained for 9-aminoacridine as a matrix. Conclusions The instrument response depends on fatty acyls composition of PCs. This dependence must be taken into account upon the quantitative MALDI-MS analysis of phospholipids. MALDI matrix must also be chosen with care to avoid distortion of estimation of lipid concentrations based on peak intensities. Authors acknowledge the support of projects No. LL1302 (ERC CZ) and No. CZ.1.07/2.3.00/30.0021 (MSMT CR). Novel Aspect Comprehensive study of PCs composition and concentration influence on the response of mass spectrometer with Orbitrap mass analyzer is provided. The effect of MALDI matrix on the response is considered as well. (en)
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| Title
| - Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS
- Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS (en)
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| skos:prefLabel
| - Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS
- Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS (en)
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| skos:notation
| - RIV/00216275:25310/14:39899345!RIV15-MSM-25310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(EE2.3.30.0021), P(LL1302)
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/14:39899345
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - MALDI, phosphatidylcholine, lipids (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Holčapek, Michal
- Lísa, Miroslav
- Chagovets, Vitaliy Viktorovich
|
| http://localhost/t...ganizacniJednotka
| |
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