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  • Conditions of hydrophilic interaction liquid chromatopraphy of labelled oligosaccharides (columns, gradient profiles, mobile phase flow and additives) were optimized with the aim of coupling it to the capillary zone electrophoresis in two-dimensional system. Different common derivatization reagents used for fluorescent labeling of oligosaccharides including 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) we compared. For a separation of labelled oligosaccharides, microcolumns with different stationary phases were tested (Silica gel, Amine, TSK gel amide-80 and GlycanPac stationary phases) Mass spectrometer was used for detection.
  • Conditions of hydrophilic interaction liquid chromatopraphy of labelled oligosaccharides (columns, gradient profiles, mobile phase flow and additives) were optimized with the aim of coupling it to the capillary zone electrophoresis in two-dimensional system. Different common derivatization reagents used for fluorescent labeling of oligosaccharides including 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) we compared. For a separation of labelled oligosaccharides, microcolumns with different stationary phases were tested (Silica gel, Amine, TSK gel amide-80 and GlycanPac stationary phases) Mass spectrometer was used for detection. (en)
Title
  • Retention characteristics of labelled oligosaccharides in hydrophilic interaction liquid chromatography
  • Retention characteristics of labelled oligosaccharides in hydrophilic interaction liquid chromatography (en)
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  • Retention characteristics of labelled oligosaccharides in hydrophilic interaction liquid chromatography
  • Retention characteristics of labelled oligosaccharides in hydrophilic interaction liquid chromatography (en)
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  • RIV/00216275:25310/14:39898288!RIV15-MSM-25310___
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  • P(GA14-06319S), S
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  • 42543
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  • RIV/00216275:25310/14:39898288
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  • hydrophilic interaction liquid chromatography; labelled oligosaccharides; Retention characteristics (en)
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  • [9BA27D7F589E]
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  • Brno
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  • Brno
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  • CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis
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  • Fischer, Jan
  • Česla, Petr
  • Vaňková, Nikola
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number of pages
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  • Ústav analytické chemie AV ČR, v. v. i.
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  • 978-80-904959-2-0
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  • 25310
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