About: Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions

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About: Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A method for determination of 18 flavonoid aglycones, glycosides and phenolic acids using micellar electrokinetic chromatography has been developed. Sodium decyl sulphate was used as pseudostationary phase at the concentration of 36 mmol L-1 and addition of cyclodextrins up to the 5 mmol L-1 was tested for improvement of the separation of isomeric compounds. In the electrolyte containing heptakis(2,6-di-O-methyl)-beta-cyclodextrin, three isomers of chlorogenic acid (chlorogenic, neo- and cryptochlorogenic acid) were successfully baseline separated. The quantitative parameters of the method were determined including limits of quantification, which were in the range of 0.15-1.4 ug mL-1, together with the linearity of calibration curves. The method was successfully applied for the analyses of water infusions of elderflower samples and the statistically significant differences were found between the concentrations of polyphenols depending on the sample harvest location. A method for determination of 18 flavonoid aglycones, glycosides and phenolic acids using micellar electrokinetic chromatography has been developed. Sodium decyl sulphate was used as pseudostationary phase at the concentration of 36 mmol L-1 and addition of cyclodextrins up to the 5 mmol L-1 was tested for improvement of the separation of isomeric compounds. In the electrolyte containing heptakis(2,6-di-O-methyl)-beta-cyclodextrin, three isomers of chlorogenic acid (chlorogenic, neo- and cryptochlorogenic acid) were successfully baseline separated. The quantitative parameters of the method were determined including limits of quantification, which were in the range of 0.15-1.4 ug mL-1, together with the linearity of calibration curves. The method was successfully applied for the analyses of water infusions of elderflower samples and the statistically significant differences were found between the concentrations of polyphenols depending on the sample harvest location. (en)
Title	Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions (en)
skos:prefLabel	Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions Development of Micellar Electrokinetic Chromatographic Method for Determination of Polyphenols in Elder Infusions (en)
skos:notation	RIV/00216275:25310/14:39898282!RIV15-MSM-25310___
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Fischer, Jan Česla, Petr Česlová, Lenka Váňová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	11013
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/14:39898282
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	elderflower; flavonoids; phenolic acids; micellar electrokinetic chromatography (en)
http://linked.open.../riv/klicoveSlovo	phenolic acids micellar electrokinetic chromatography flavonoids elderflower
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1EEC97EBE11A]
http://linked.open...i/riv/nazevZdroje	Chemické listy
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	108
http://linked.open...iv/tvurceVysledku	Fischer, Jan Česla, Petr Česlová, Lenka Váňová, Jana
http://linked.open...ain/vavai/riv/wos	000346508500008
issn	0009-2770
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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