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| rdf:type
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| Description
| - Tau protein plays a crucial role in the neuronal cytoskeleton stabilization. Under the pathological conditions, it can be abnormally phosphorylated which leads to the aggregation and formation of neurofibrillary tangles representing pathological hallmark of Alzheimer's disease (AD). For its association with neurodegenerative diseases, tau protein is intensively studied in various diagnostic and therapeutic applications. Since there is no standard of tau protein involving essential post-translational modifications, it is often necessary to purify it directly from cerebrospinal fluid (CSF) or blood of healthy or AD clinical signs exhibiting organism. The immunomagnetic purification based on the isolation of the target protein using a specific antibody bound to a magnetic carrier is the most effective tool for this purpose. High quality antibodies are the main prerequisite of successful purification, but many commercial antibodies do not comply with the challenging requirements for the immunosorbent preparation. In this work, we compared four different anti-tau monoclonal antibodies currently available on the market (clones HT7, BT2, 8F10, 7E5). The evaluation criteria were set along the intended use for the preparation of specific magnetic immunosorbent subsequently applicable for the native tau protein purification. We evaluated the characteristics declared by producers as specificity, purity and homogeneity. We also tested the binding affinity and IgG stability during the covalent immobilization to the surface of magnetic microparticles and during the immunoprecipitation of intact tau protein or tryptic tau fragments. The results are summarized and discussed here.
- Tau protein plays a crucial role in the neuronal cytoskeleton stabilization. Under the pathological conditions, it can be abnormally phosphorylated which leads to the aggregation and formation of neurofibrillary tangles representing pathological hallmark of Alzheimer's disease (AD). For its association with neurodegenerative diseases, tau protein is intensively studied in various diagnostic and therapeutic applications. Since there is no standard of tau protein involving essential post-translational modifications, it is often necessary to purify it directly from cerebrospinal fluid (CSF) or blood of healthy or AD clinical signs exhibiting organism. The immunomagnetic purification based on the isolation of the target protein using a specific antibody bound to a magnetic carrier is the most effective tool for this purpose. High quality antibodies are the main prerequisite of successful purification, but many commercial antibodies do not comply with the challenging requirements for the immunosorbent preparation. In this work, we compared four different anti-tau monoclonal antibodies currently available on the market (clones HT7, BT2, 8F10, 7E5). The evaluation criteria were set along the intended use for the preparation of specific magnetic immunosorbent subsequently applicable for the native tau protein purification. We evaluated the characteristics declared by producers as specificity, purity and homogeneity. We also tested the binding affinity and IgG stability during the covalent immobilization to the surface of magnetic microparticles and during the immunoprecipitation of intact tau protein or tryptic tau fragments. The results are summarized and discussed here. (en)
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| Title
| - QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN
- QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN (en)
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| skos:prefLabel
| - QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN
- QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN (en)
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| skos:notation
| - RIV/00216275:25310/14:39898080!RIV15-MSM-25310___
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(7E12083), P(EE2.3.30.0021)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/14:39898080
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - monoclonal antibody; immunomagnetic purification; Alzheimer's disease; Tau protein (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Řípová, Daniela
- Bílková, Zuzana
- Jankovičová, Barbora
- Hromádková, Lenka
- Kupčík, Rudolf
- Kašparová, Jitka
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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