About: Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Selecting the right monoclonal antibody (mAb) for an immunoaffinity-based application can be tricky, as many mAb producers offer a wide range of mAb clones against molecular structures of interest. Since there are significant differences in the quality of mAb clones, and particularly in their binding activity, an easy method for quick and low-cost comparison of various mAb clones was developed. The dot-ELISA affinity test is a simple, versatile and instrumentally no demanding technique, since it requires no expensive equipment (such as an ELISA reader or chemiluminescence/fluorescence imaging system) and can be performed in any biochemical laboratory. This method is based on a previously described dot-ELISA technique that is improved with a chaotropic step using different concentrations of ammonium thiocyanate in the range 0-2 M. In this work, the dot-ELISA affinity test was optimized on Abeta peptide as antigen and anti-Abeta mAb. Such protocol was then applied to a panel of eight anti-EpCAM (epithelial cell adhesion molecule) mAbs which should be subsequently used for preparation of magnetic immunosorbent to capture circulating tumor cells (CTCs). Selecting the right monoclonal antibody (mAb) for an immunoaffinity-based application can be tricky, as many mAb producers offer a wide range of mAb clones against molecular structures of interest. Since there are significant differences in the quality of mAb clones, and particularly in their binding activity, an easy method for quick and low-cost comparison of various mAb clones was developed. The dot-ELISA affinity test is a simple, versatile and instrumentally no demanding technique, since it requires no expensive equipment (such as an ELISA reader or chemiluminescence/fluorescence imaging system) and can be performed in any biochemical laboratory. This method is based on a previously described dot-ELISA technique that is improved with a chaotropic step using different concentrations of ammonium thiocyanate in the range 0-2 M. In this work, the dot-ELISA affinity test was optimized on Abeta peptide as antigen and anti-Abeta mAb. Such protocol was then applied to a panel of eight anti-EpCAM (epithelial cell adhesion molecule) mAbs which should be subsequently used for preparation of magnetic immunosorbent to capture circulating tumor cells (CTCs). (en)
Title	Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies (en)
skos:prefLabel	Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies (en)
skos:notation	RIV/00216275:25310/13:39896743!RIV14-GA0-25310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(7E09080), P(7E09109), P(7E12053), P(7E12083), P(EE2.3.30.0021), P(GAP206/12/0381)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Bílková, Zuzana Jankovičová, Barbora Svobodová, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	70495
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/13:39896743
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Abeta peptides; EpCAM; Chaotropic elution; Monoclonal antibody; Affinity; Dot-ELISA; Dot blot (en)
http://linked.open.../riv/klicoveSlovo	Monoclonal antibody Abeta peptides Chaotropic elution Dot blot Dot-ELISA EpCAM Affinity
http://linked.open...odStatuVydavatele	RO - Rumunsko
http://linked.open...ontrolniKodProRIV	[2465348E4A98]
http://linked.open...i/riv/nazevZdroje	Journal of Analytical and Bioanalytical Techniques
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells Development of ultrasensitive electrochemical immunosensors for early detection of clinically significant biomarkers of malignant diseases Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells NAnosystems for the early DIagnosis of NEurodegenerative Diseases Strenthening of Research and Development Teams at the University of Pardubice Nanosystems for early Diagnosis of Neurodegenerative Diseases
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Horák, Daniel Svobodová, Zuzana Bílková, Zuzana Jankovičová, Barbora
issn	2155-9872
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.4172/2155-9872.1000168
http://localhost/t...ganizacniJednotka	25310

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