About: An assay of creatine in human serum using an HPLC with UV detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An ion-exchange high-performance liquid chromatography with ultraviolet detection for the determination of creatine in human serum has been developed. The human serum was deproteinized with perchloric acid. For the separation, a cation-exchange column LC-SCX, 250 mm x 4.6 mm, 5 um, was used. The mixture of ethanol and 10mmol l-1 ammonium acetate (15:85, v/v), pH 4.00+/-0.05 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries of spiked serum samples were between 97.1% and 103.5%. The calibration curve was linear in the whole range tested. The limit of quantification was 2 umol l-1. The preliminary reference ranges of serum creatine in a group of blood donors are 17-89 umol l-1 for women and 21-97 umol l-1 for men. An ion-exchange high-performance liquid chromatography with ultraviolet detection for the determination of creatine in human serum has been developed. The human serum was deproteinized with perchloric acid. For the separation, a cation-exchange column LC-SCX, 250 mm x 4.6 mm, 5 um, was used. The mixture of ethanol and 10mmol l-1 ammonium acetate (15:85, v/v), pH 4.00+/-0.05 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries of spiked serum samples were between 97.1% and 103.5%. The calibration curve was linear in the whole range tested. The limit of quantification was 2 umol l-1. The preliminary reference ranges of serum creatine in a group of blood donors are 17-89 umol l-1 for women and 21-97 umol l-1 for men. (en)
Title	An assay of creatine in human serum using an HPLC with UV detection An assay of creatine in human serum using an HPLC with UV detection (en)
skos:prefLabel	An assay of creatine in human serum using an HPLC with UV detection An assay of creatine in human serum using an HPLC with UV detection (en)
skos:notation	RIV/00216275:25310/13:39896728!RIV14-MSM-25310___
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	prosinec
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kanďár, Roman Štramová, Xenie Drábková, Petra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	60309
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/13:39896728
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	reference values; ion-exchange chromatography; creatine (en)
http://linked.open.../riv/klicoveSlovo	creatine ion-exchange chromatography reference values
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[738CD2032558]
http://linked.open...i/riv/nazevZdroje	Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Kanďár, Roman Drábková, Petra Štramová, Xenie
issn	1211-5541
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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