About: Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection

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About: Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine. A relative simple method for the simultaneous determination of epinephrine, norepinephrine and dopamine in urine is presented. We have used a high-performance liquid chromatography (HPLC) with coulometric electrochemical detection. Urine samples pretreatment by a solid-phase extraction were injected directly into the HPLC system and were eluted isocratically on a Discovery HS C18, 250 x 4 mm i.d., 5 um, analytical column. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked urine samples were at intervals 78.2-88.0% for epinephrine, 80.0-93.0% for norepinephrine, and 96.4-107.2% for dopamine. The linear range is 2-200 ug l-1, with a detection limit of 0.6 ug l-1 (signal-to-noise ratio = 3) for epinephrine, 5-500 ug l-1, with a detection limit of 1.5 ug l-1 for norepinephrine, and 20-2000 ug l-1, with a detection limit of 6 ug l-1 for dopamine. (en)
Title	Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection (en)
skos:prefLabel	Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection Determination of epinephrine, norepinephrine and dopamine in human urine using high-performance liquid chromatography with coulometric detection (en)
skos:notation	RIV/00216275:25310/13:39896727!RIV14-MSM-25310___
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	prosinec
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kanďár, Roman Štramová, Xenie Drábková, Petra Žáková, Pavla Bencová, Soňa
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	68871
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/13:39896727
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	solid-phase extraction; coulometric electrochemical detection; liquid chromatography; dopamine; norepinephrine; epinephrine (en)
http://linked.open.../riv/klicoveSlovo	liquid chromatography coulometric electrochemical detection solid-phase extraction epinephrine dopamine norepinephrine
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[47682EFFAC1D]
http://linked.open...i/riv/nazevZdroje	Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Kanďár, Roman Drábková, Petra Štramová, Xenie Žáková, Pavla Bencová, Soňa
issn	1211-5541
number of pages	14 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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