About: Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm     Goto   Sponge   NotDistinct   Permalink

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  • This paper concerns the formation of biofilm in bacteria of the genus Arcobacter. A multiplex polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA), first for analysis of strains of the genus Arcobacter from a collection, and then applied to samples of prepared biofilms. The results of the study indicate considerable variability among species of bacteria within the genus Arcobacter. The EMA?PMA PCR method can distinguish viable cells from dead cells and is therefore suitable for determining the viability of cells.
  • This paper concerns the formation of biofilm in bacteria of the genus Arcobacter. A multiplex polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA), first for analysis of strains of the genus Arcobacter from a collection, and then applied to samples of prepared biofilms. The results of the study indicate considerable variability among species of bacteria within the genus Arcobacter. The EMA?PMA PCR method can distinguish viable cells from dead cells and is therefore suitable for determining the viability of cells. (en)
Title
  • Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm
  • Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm (en)
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  • Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm
  • Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm (en)
skos:notation
  • RIV/00216275:25310/13:39896521!RIV14-MSM-25310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, S
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 90153
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/13:39896521
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  • Arcobacter; biofilm; PCR; ethidium monoazide; propidium monoazide (en)
http://linked.open.../riv/klicoveSlovo
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  • CA - Kanada
http://linked.open...ontrolniKodProRIV
  • [4C6BB0BA9BE8]
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  • Canadian Journal of Microbiology
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  • 59
http://linked.open...iv/tvurceVysledku
  • Moťková, Petra
  • Vytřasová, Jarmila
  • Hrušková, Lucie
http://linked.open...ain/vavai/riv/wos
  • 000328471300004
issn
  • 0008-4166
number of pages
http://bibframe.org/vocab/doi
  • 10.1139/cjm-2013-0635
http://localhost/t...ganizacniJednotka
  • 25310
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