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  • The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (+4 and +20?C) were selected. In three short-term studies in which the storage time varied between 3 and 48 hrs the performance of these assays were tested on human serum samples. The general conclusion is that most assays performed well and gave stable results during 2 days of storage of the samples at both temperatures. Only the FRAP and TAS assays showed a small deviation at some storage conditions. In conclusion, handling of serum samples at +4 and +20?C during short-time periods did not affect the quality and performance of the oxidation and antioxidant assays during day-to-day analyses.
  • The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (+4 and +20?C) were selected. In three short-term studies in which the storage time varied between 3 and 48 hrs the performance of these assays were tested on human serum samples. The general conclusion is that most assays performed well and gave stable results during 2 days of storage of the samples at both temperatures. Only the FRAP and TAS assays showed a small deviation at some storage conditions. In conclusion, handling of serum samples at +4 and +20?C during short-time periods did not affect the quality and performance of the oxidation and antioxidant assays during day-to-day analyses. (en)
Title
  • Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum
  • Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum (en)
skos:prefLabel
  • Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum
  • Short-term stability of biomarkers of oxidative stress and antioxidant status in human serum (en)
skos:notation
  • RIV/00216275:25310/13:39896517!RIV14-MSM-25310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I
http://linked.open...iv/cisloPeriodika
  • 2013
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 104980
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/13:39896517
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • serum; antioxidant status; biomarkers of oxidative stress; Short-term stability (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • EG - Egyptská arabská republika
http://linked.open...ontrolniKodProRIV
  • [CA621B2D0311]
http://linked.open...i/riv/nazevZdroje
  • ISRN Biomarkers
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2013
http://linked.open...iv/tvurceVysledku
  • Mužáková, Vladimíra
  • Viezeliene, D.
  • Beekhof, P. K
  • Cremers, JWJM
  • Jansen, EHJM
  • Skalicky, J.
issn
  • 2314-6265
number of pages
http://bibframe.org/vocab/doi
  • 10.1155/4174
http://localhost/t...ganizacniJednotka
  • 25310
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