About: New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors

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About: New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.znaturforsch.com/ac/v68c/c68c.htm#No3
Description	A new and simple analytical method is described for the determination of the IC50 values of the inhibitors of the hydrolysis of acetylcholine (ACh) or acetylthiocholine (ATCh) by cholinesterases. The method is based on monitoring the time course of the pH value during the uninhibited and inhibited reaction. It requires only a pH meter with a suitable pH measuring cell and a small thermostated stirred batch reactor. The method has been validated for twelve different types of cholinesterase inhibitors. The determined IC50 values are comparable to those obtained by independent, more complicated, and expensive methods (Ellman's and pH-stat). A new and simple analytical method is described for the determination of the IC50 values of the inhibitors of the hydrolysis of acetylcholine (ACh) or acetylthiocholine (ATCh) by cholinesterases. The method is based on monitoring the time course of the pH value during the uninhibited and inhibited reaction. It requires only a pH meter with a suitable pH measuring cell and a small thermostated stirred batch reactor. The method has been validated for twelve different types of cholinesterase inhibitors. The determined IC50 values are comparable to those obtained by independent, more complicated, and expensive methods (Ellman's and pH-stat). (en)
Title	New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors (en)
skos:prefLabel	New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors New Method for the Determination of the Half Inhibition Concentration (IC50) of Cholinesterase Inhibitors (en)
skos:notation	RIV/00216275:25310/13:39896446!RIV14-MSM-25310___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Čegan, Alexander Komers, Karel Kovářová, Markéta Pařík, Patrik Štěpánková, Šárka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	91622
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/13:39896446
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	determination; IC50; cholinesterase Inhibition (en)
http://linked.open.../riv/klicoveSlovo	cholinesterase Inhibition IC50 determination
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[DE92A2A130BA]
http://linked.open...i/riv/nazevZdroje	Zeitschrift fuer Naturforschung C-A Journal of Biosciences
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	68
http://linked.open...iv/tvurceVysledku	Pařík, Patrik Čegan, Alexander Komers, Karel Kovářová, Markéta Štěpánková, Šárka
http://linked.open...ain/vavai/riv/wos	000322090700009
issn	0939-5075
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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