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Description
  • A method is described here for the determination of total glutathione and glutathione disulfide in human whole blood and plasma with a suitable sample preparation procedure to prevent glutathione oxidation and glutathione disulfide hydrolysis. Whole blood samples were obtained from a group of blood donors. After adequate sample preparation, the samples were derivatized with ortho-phthaldialdehyde to form a stable, highly fluorescent tricyclic derivative. Reverse-phase column chromatography was used for the separation and fluorimetric detection to monitor the effluent. The analytical performance of this method was satisfactory for the determination of total glutathione and glutathione disulfide. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 96.1% (CV 1.4%) and 104.3% (CV 2.4%) for whole blood, 92.3% (CV 2.4%) and 107.4% (CV 2.6%) for plasma. We found no significant differences in both total glutathione and glutathione disulfide concentration between the women and men. We developed a selective high-performance liquid chromatography method for the determination of total glutathione and glutathione disulfide in human whole blood and plasma. Our sophisticated sample preparation procedure prevents significant glutathione oxidation and glutathione disulfide hydrolysis.
  • A method is described here for the determination of total glutathione and glutathione disulfide in human whole blood and plasma with a suitable sample preparation procedure to prevent glutathione oxidation and glutathione disulfide hydrolysis. Whole blood samples were obtained from a group of blood donors. After adequate sample preparation, the samples were derivatized with ortho-phthaldialdehyde to form a stable, highly fluorescent tricyclic derivative. Reverse-phase column chromatography was used for the separation and fluorimetric detection to monitor the effluent. The analytical performance of this method was satisfactory for the determination of total glutathione and glutathione disulfide. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 96.1% (CV 1.4%) and 104.3% (CV 2.4%) for whole blood, 92.3% (CV 2.4%) and 107.4% (CV 2.6%) for plasma. We found no significant differences in both total glutathione and glutathione disulfide concentration between the women and men. We developed a selective high-performance liquid chromatography method for the determination of total glutathione and glutathione disulfide in human whole blood and plasma. Our sophisticated sample preparation procedure prevents significant glutathione oxidation and glutathione disulfide hydrolysis. (en)
Title
  • An assay of total glutathione and glutathione disulfide in human whole blood and plasma using a high-performance liquid chromatography with fluorescence detection
  • An assay of total glutathione and glutathione disulfide in human whole blood and plasma using a high-performance liquid chromatography with fluorescence detection (en)
skos:prefLabel
  • An assay of total glutathione and glutathione disulfide in human whole blood and plasma using a high-performance liquid chromatography with fluorescence detection
  • An assay of total glutathione and glutathione disulfide in human whole blood and plasma using a high-performance liquid chromatography with fluorescence detection (en)
skos:notation
  • RIV/00216275:25310/13:39896347!RIV14-MSM-25310___
http://linked.open...avai/riv/aktivita
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  • I, S
http://linked.open...iv/cisloPeriodika
  • 14
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 60310
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  • RIV/00216275:25310/13:39896347
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  • protein precipitants; oxidative stress; ortho-phthaldialdehyde; high-performance liquid chromatography with fluorescence detection; glutathione disulfide; glutathione; dithiothreitol (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [041191BC71A6]
http://linked.open...i/riv/nazevZdroje
  • Journal of Liquid Chromatography & Related Technologies
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 36
http://linked.open...iv/tvurceVysledku
  • Kanďár, Roman
  • Vrbová, Martina
  • Čandová, Jarmila
http://linked.open...ain/vavai/riv/wos
  • 000319553100009
issn
  • 1082-6076
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  • 25310
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