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  • In this article, a method for the analysis of N-linked glycans derived from glycoproteins is described. Complex mixtures of APTS-labeled neutral and acidic N-glycans were resolved using capillary zone electrophoresis coupled with laser-induced fluorescence detector. All separations were carried out on a polyacrylamide coated capillary with reduced electroosmotic flow. N-glycan pools released from several standard glycoproteins were analyzed in order to generate a migration time database used for the identification of N-glycans released from more complicated samples. In addition, enzymatic digestions using specific and non-specific exoglycosidases were used to positively identify glycan isomeric structures.
  • In this article, a method for the analysis of N-linked glycans derived from glycoproteins is described. Complex mixtures of APTS-labeled neutral and acidic N-glycans were resolved using capillary zone electrophoresis coupled with laser-induced fluorescence detector. All separations were carried out on a polyacrylamide coated capillary with reduced electroosmotic flow. N-glycan pools released from several standard glycoproteins were analyzed in order to generate a migration time database used for the identification of N-glycans released from more complicated samples. In addition, enzymatic digestions using specific and non-specific exoglycosidases were used to positively identify glycan isomeric structures. (en)
Title
  • Comprehensive Profiling of APTS-Labeled N-Glycans Using Capillary Zone Electrophoresis / Laser Induced Fluorescence
  • Comprehensive Profiling of APTS-Labeled N-Glycans Using Capillary Zone Electrophoresis / Laser Induced Fluorescence (en)
skos:prefLabel
  • Comprehensive Profiling of APTS-Labeled N-Glycans Using Capillary Zone Electrophoresis / Laser Induced Fluorescence
  • Comprehensive Profiling of APTS-Labeled N-Glycans Using Capillary Zone Electrophoresis / Laser Induced Fluorescence (en)
skos:notation
  • RIV/00216275:25310/12:39895661!RIV13-MSM-25310___
http://linked.open...avai/riv/aktivita
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  • I
http://linked.open...vai/riv/dodaniDat
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  • 128249
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/12:39895661
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  • APTS.; N-Glycans; Glycoproteins; Laser induced fluorescence; Capillary zone electrophoresis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [C292CDFE5051]
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  • Pardubice
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  • Pardubice
http://linked.open...i/riv/nazevZdroje
  • Sensing in Electroanalysis Vol. 7
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
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  • Jandera, Pavel
  • Staněk, Václav
  • Novotný, Milos V.
  • Starkey, Jason A.
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http://linked.open.../riv/zahajeniAkce
number of pages
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  • Univerzita Pardubice
https://schema.org/isbn
  • 978-80-7395-563-2
http://localhost/t...ganizacniJednotka
  • 25310
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