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Description
| - Determination of amyloid beta (Abeta) isoforms - and in particular the proportion of the Abeta 1-42 isoform - in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease (AD) might help in early diagnosis and treatment of that illness. Due to the low concentration of Abeta peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (mýIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Abeta antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the proper beads and anti-Abeta antibodies, as well as optimizing the immobilization technique and mýIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Abeta IS for mýIP and preconcentration of 5 Abeta peptides under optimized conditions using various subsequent analytical methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), capillary electrophoresis (CE), microchip electrophoresis (MCE), and immunoblotting. Synthetic Abeta peptides samples prepared in buffer and spiked in human CSF were analyzed. Finally, on-chip immunoprecipitation of Abeta peptides in human CSF sample was performed.
- Determination of amyloid beta (Abeta) isoforms - and in particular the proportion of the Abeta 1-42 isoform - in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease (AD) might help in early diagnosis and treatment of that illness. Due to the low concentration of Abeta peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (mýIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Abeta antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the proper beads and anti-Abeta antibodies, as well as optimizing the immobilization technique and mýIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Abeta IS for mýIP and preconcentration of 5 Abeta peptides under optimized conditions using various subsequent analytical methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), capillary electrophoresis (CE), microchip electrophoresis (MCE), and immunoblotting. Synthetic Abeta peptides samples prepared in buffer and spiked in human CSF were analyzed. Finally, on-chip immunoprecipitation of Abeta peptides in human CSF sample was performed. (en)
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Title
| - Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers
- Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers (en)
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skos:prefLabel
| - Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers
- Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers (en)
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skos:notation
| - RIV/00216275:25310/12:39894651!RIV13-MSM-25310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/12:39894651
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - biomarker; Alzheimer's disease; amyloid beta; preconcentration; microfluidics; immunosorbent (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Svobodová, Zuzana
- Bílková, Zuzana
- Jankovičová, Barbora
- Esselmann, Hermann
- Mohamadi, Mohamad Reza
- Otto, Markus
- Taverna, Myriam
- Verpillot, Romain
- Viovy, Jean-Louis
- Wiltfang, Jens
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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http://localhost/t...ganizacniJednotka
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