About: New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.

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About: New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed. Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed. (en)
Title	New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations. New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations. (en)
skos:prefLabel	New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations. New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations. (en)
skos:notation	RIV/00216275:25310/12:39894517!RIV13-GA0-25310___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(7E09109), P(7E12053), P(GA203/09/0857), P(GAP503/10/0664), Z(AV0Z40500505), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Mikulášek, Petr Palarčík, Jiří Korecká, Lucie Bílková, Zuzana Jankovičová, Barbora Kučerová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	154065
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/12:39894517
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	2-hydroxyethyl methacrylate; glycidyl methacrylate; enzyme catalysis (en)
http://linked.open.../riv/klicoveSlovo	glycidyl methacrylate 2-hydroxyethyl methacrylate enzyme catalysis
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[67777A9BC1A8]
http://linked.open...i/riv/nazevZdroje	Macromolecular Bioscience
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells New analytical approaches for identification of proteins with significant attributes for virulence and pathogenity of bacteria Isolation, typing and the development of immunochemical and instrumetal methods for detection and characterization of Cronobacter sp. Nanosystems for early Diagnosis of Neurodegenerative Diseases
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Horák, Daniel Kučerová, Jana Mikulášek, Petr Palarčík, Jiří Bílková, Zuzana Korecká, Lucie Jankovičová, Barbora
http://linked.open...ain/vavai/riv/wos	000303661300010
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1616-5187
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/mabi.201100393
http://localhost/t...ganizacniJednotka	25310

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