About: Kinetics of In Vitro Inhibition of Acetylcholinesterase by Nineteen New Carbamates

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two series A and B of 12 and 7 new carbamate derivates were tested in vitro as acetylcholinesterase inhibitors. The tests were performed in the batch stirred reactor at 25°C, pH 8, ionic strength 0.11 M and catalytic activity of the enzyme preparation 0.14 U mL-1 of the reaction mixture. The temporal dependences of actual concentrations of acetylcholine, choline and acetic acid were determined by two independent analytical methods, HPLC and pH-stat. For all used inhibitors, the model of competitive irreversible inhibition was valid. The inhibition rate constant k3 and qualified estimation of the absolute acetylcholinesterase concentration in the reaction mixture were calculated. The partition coefficients Kow between n-octanol and water of all used inhibitors were determined. The k3 and Kow values were correlated with the Hammett and Hansch substituent constants and with the calculated docking and binding energies of the reaction between the tested inhibitors and acetylcholinesterase. Two series A and B of 12 and 7 new carbamate derivates were tested in vitro as acetylcholinesterase inhibitors. The tests were performed in the batch stirred reactor at 25°C, pH 8, ionic strength 0.11 M and catalytic activity of the enzyme preparation 0.14 U mL-1 of the reaction mixture. The temporal dependences of actual concentrations of acetylcholine, choline and acetic acid were determined by two independent analytical methods, HPLC and pH-stat. For all used inhibitors, the model of competitive irreversible inhibition was valid. The inhibition rate constant k3 and qualified estimation of the absolute acetylcholinesterase concentration in the reaction mixture were calculated. The partition coefficients Kow between n-octanol and water of all used inhibitors were determined. The k3 and Kow values were correlated with the Hammett and Hansch substituent constants and with the calculated docking and binding energies of the reaction between the tested inhibitors and acetylcholinesterase. (en)
Title	Kinetics of In Vitro Inhibition of Acetylcholinesterase by Nineteen New Carbamates Kinetics of In Vitro Inhibition of Acetylcholinesterase by Nineteen New Carbamates (en)
skos:prefLabel	Kinetics of In Vitro Inhibition of Acetylcholinesterase by Nineteen New Carbamates Kinetics of In Vitro Inhibition of Acetylcholinesterase by Nineteen New Carbamates (en)
skos:notation	RIV/00216275:25310/11:39895922!RIV13-MSM-25310___
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM0021627501), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Čegan, Alexander Komers, Karel Kovářová, Markéta Zatloukalová, Martina Pařík, Patrik
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	207420
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/11:39895922
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	docking energies; HPLC; kinetics; inhibition; carbamates; acetylcholinesterase; in vitro; enzymatic; hydrolysis; Acetylcholine (en)
http://linked.open.../riv/klicoveSlovo	Acetylcholine inhibition kinetics HPLC acetylcholinesterase hydrolysis in vitro carbamates docking energies enzymatic
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E11956F65797]
http://linked.open...i/riv/nazevZdroje	Current Enzyme Inhibition
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Pařík, Patrik Čegan, Alexander Khan, Mahmud T.H. Komers, Karel Kovářová, Markéta Zatloukalová, Martina
http://linked.open...n/vavai/riv/zamer	Aimed preparation of special compounds and materials and investigation of their physical and chemical properties and supramolecular structures Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1573-4080
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.2174/157340811799860560
http://localhost/t...ganizacniJednotka	25310

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