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  • Glutathione reductase is a crucial enzyme for maintaining of intracellular glutathione levels. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to the reduced form (GSH). The aim of our study was to estimate a possible inhibitory effect on glutathione reductase activity in the presence of two substances that cause glutathione depletion - ethacrynic acid and diethyl maleate. We also tested glutathione as a possible inhibitor. GR activity was determined using spectrophotometric method based on measurement of absorbance decline (lambda = 340 nm) due to oxidation of NADPH. We found dose dependent inhibitory effect through reduced glutathione - the presence of 10 mM GSH caused a decrease in enzyme activity by 60%. We conclude that our finding of inhibitory effect in presence of glutathione is of great importance since the GSH levels are very high in the cells.
  • Glutathione reductase is a crucial enzyme for maintaining of intracellular glutathione levels. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to the reduced form (GSH). The aim of our study was to estimate a possible inhibitory effect on glutathione reductase activity in the presence of two substances that cause glutathione depletion - ethacrynic acid and diethyl maleate. We also tested glutathione as a possible inhibitor. GR activity was determined using spectrophotometric method based on measurement of absorbance decline (lambda = 340 nm) due to oxidation of NADPH. We found dose dependent inhibitory effect through reduced glutathione - the presence of 10 mM GSH caused a decrease in enzyme activity by 60%. We conclude that our finding of inhibitory effect in presence of glutathione is of great importance since the GSH levels are very high in the cells. (en)
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  • Assessment of glutathione reductase inhibition
  • Assessment of glutathione reductase inhibition (en)
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  • Assessment of glutathione reductase inhibition
  • Assessment of glutathione reductase inhibition (en)
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  • RIV/00216275:25310/11:39892670!RIV12-MSM-25310___
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  • RIV/00216275:25310/11:39892670
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  • spectrophotometry; glutathione; Glutathione reductase (en)
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  • Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
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  • 17
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  • Nýdlová, Erika
  • Roušar, Tomáš
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