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rdf:type
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Description
| - Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization of the PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time used and tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further development and adaptation for flowing streams are also outlined.
- Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization of the PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time used and tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further development and adaptation for flowing streams are also outlined. (en)
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Title
| - A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film
- A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film (en)
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skos:prefLabel
| - A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film
- A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film (en)
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skos:notation
| - RIV/00216275:25310/11:39892617!RIV12-MSM-25310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(LC06035), Z(MSM0021627502)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/11:39892617
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Glucose; Glucose oxidase; Polyaniline film; Carbon paste; Biosensor (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - CS - Státní svaz Srbska a Černé Hory
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - International Journal of Electrochemical Science
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kalcher, Kurt
- Stočes, Matěj
- Vytřas, Karel
- Švancara, Ivan
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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