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  • Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase liquid chromatography separations of polar, weakly acidic or basic samples. In principle, this separation mode can be characterized as normal-phase chromatography on polar columns in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). Highly organic HILIC mobile phases usually enhance ionization in the electrospray ion source of a mass spectrometer, in comparison to mobile phases with higher concentrations of water generally used in reversed-phase (RP) LC separations of polar or ionic compounds, which is another reason for increasing popularity of this technique. Various columns can be used in the HILIC mode for separations of peptides, proteins, oligosaccharides, drugs, metabolites and various natural compounds.
  • Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase liquid chromatography separations of polar, weakly acidic or basic samples. In principle, this separation mode can be characterized as normal-phase chromatography on polar columns in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). Highly organic HILIC mobile phases usually enhance ionization in the electrospray ion source of a mass spectrometer, in comparison to mobile phases with higher concentrations of water generally used in reversed-phase (RP) LC separations of polar or ionic compounds, which is another reason for increasing popularity of this technique. Various columns can be used in the HILIC mode for separations of peptides, proteins, oligosaccharides, drugs, metabolites and various natural compounds. (en)
Title
  • Hydrophilic Interaction Chromatography - An Excellent Method for Separation of Polar Samples
  • Hydrophilic Interaction Chromatography - An Excellent Method for Separation of Polar Samples (en)
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  • Hydrophilic Interaction Chromatography - An Excellent Method for Separation of Polar Samples
  • Hydrophilic Interaction Chromatography - An Excellent Method for Separation of Polar Samples (en)
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  • RIV/00216275:25310/11:39892521!RIV12-GA0-25310___
http://linked.open...avai/riv/aktivita
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  • P(GA203/07/0641), Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat
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  • 203152
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  • RIV/00216275:25310/11:39892521
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  • Polar samples; Phase systems in HILIC; HILIC (en)
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  • [51CD421CD1C0]
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  • Urbana
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  • Extreme Chromatography: Faster, Hotter, Smaller
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  • Jandera, Pavel
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  • AOCS Press
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  • 978-1-893997-66-0
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  • 25310
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