About: Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work, we describe a new method for the characterization of the lipid composition in various tissues, using off-line two-dimensional coupling of HILIC and RP-HPLC coupled to ESI and APCI mass spectrometry. In the first dimension the total lipid extracts are fractioned using HILIC into individual lipid classes. In total, 19 lipid classes (+3 regioisomeric pairs) that cover a wide range of polarities are separated in one analytical run, which is the highest number of analyzed lipid classes reported so far. The lysophospholipid regioisomers are also separated in HILIC mode followed by the identification based on the characteristic ESI mass spectra. The collected fractions of the various lipid classes are further separated in the RP mode, which offers an excellent resolution of the individual lipid species. Their ESI or APCI mass spectra give correct information on the fatty acid composition and on the individual regioisomeric positions on the glycerol skeleton. In this work, we describe a new method for the characterization of the lipid composition in various tissues, using off-line two-dimensional coupling of HILIC and RP-HPLC coupled to ESI and APCI mass spectrometry. In the first dimension the total lipid extracts are fractioned using HILIC into individual lipid classes. In total, 19 lipid classes (+3 regioisomeric pairs) that cover a wide range of polarities are separated in one analytical run, which is the highest number of analyzed lipid classes reported so far. The lysophospholipid regioisomers are also separated in HILIC mode followed by the identification based on the characteristic ESI mass spectra. The collected fractions of the various lipid classes are further separated in the RP mode, which offers an excellent resolution of the individual lipid species. Their ESI or APCI mass spectra give correct information on the fatty acid composition and on the individual regioisomeric positions on the glycerol skeleton. (en)
Title	Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry (en)
skos:prefLabel	Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry (en)
skos:notation	RIV/00216275:25310/11:39892094!RIV12-GA0-25310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP203/09/P249), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	srpen
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Lísa, Miroslav Holčapek, Michal Cífková, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	209655
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/11:39892094
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HILIC; lipidsregioisomers; two-dimensional liquid chromatography; Lipidomics (en)
http://linked.open.../riv/klicoveSlovo	Lipidomics lipidsregioisomers two-dimensional liquid chromatography HILIC
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[25A117B3C65C]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	New stationary Phases for Separation of Lipids
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	1218
http://linked.open...iv/tvurceVysledku	Holčapek, Michal Lísa, Miroslav Cífková, Eva
http://linked.open...ain/vavai/riv/wos	000293432200015
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	0021-9673
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.chroma.2011.05.081
http://localhost/t...ganizacniJednotka	25310

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