About: Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The analytical method based on the high-per formance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin,5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL-1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43 % of DPPH radical inhibition for PFE method and from 76.01 to 89.94 % in the case of USE method. The analytical method based on the high-per formance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin,5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL-1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43 % of DPPH radical inhibition for PFE method and from 76.01 to 89.94 % in the case of USE method. (en)
Title	Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity (en)
skos:prefLabel	Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity (en)
skos:notation	RIV/00216275:25310/10:39881939!RIV11-GA0-25310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/08/1536), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Dobiáš, Petr Adam, Martin Pavlíková, Petra Ventura, Karel Eisner, Aleš Švecová, Blanka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	251502
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/10:39881939
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	High-Performance Liquid Chromatography; Antioxidant Capacity; Antioxidants; Ultrasonic Extraction; Pressurised Fluid Extraction (en)
http://linked.open.../riv/klicoveSlovo	High-Performance Liquid Chromatography Antioxidants Antioxidant Capacity Pressurised Fluid Extraction Ultrasonic Extraction
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[726DC51E2C08]
http://linked.open...i/riv/nazevZdroje	Central European Journal of Chemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Progressive methods in chemical, food and clinical analysis
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Adam, Martin Dobiáš, Petr Pavlíková, Petra Ventura, Karel Eisner, Aleš Beňová, Blanka
http://linked.open...ain/vavai/riv/wos	000276548100012
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1895-1066
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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