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  • The determination of selected porphyrins, especially of coproporphyrin I and III is presently the most reliable direct approach to the screening of porphyrias. An HPLC method for the simultaneous measurement of coproporphyrin I and III in urine samples has been developed and evaluated. Separation of coproporphyrin I and III from interfering substances was achieved on reversed phase HPLC with gradient elution and fluorescence detection. Analytical performance of this method is satisfactory for both coproporphyrin I and coproporphyrin III: the intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked urine were between 85.0 and 101.1 %. The limit of detection was 3.0 and 2.3 ug/L, respectively. The preliminary reference ranges of coproporphyrin I and III in a group of donors are 5.0 51.6 ug/L (0.59 3.20 ug/mmol of creatinine) and 14.9 65.4 ug/L (0.98 8.21 ug/mmol of creatinine). The presented method is inexpensive and suitable for screening of porphyrias.
  • The determination of selected porphyrins, especially of coproporphyrin I and III is presently the most reliable direct approach to the screening of porphyrias. An HPLC method for the simultaneous measurement of coproporphyrin I and III in urine samples has been developed and evaluated. Separation of coproporphyrin I and III from interfering substances was achieved on reversed phase HPLC with gradient elution and fluorescence detection. Analytical performance of this method is satisfactory for both coproporphyrin I and coproporphyrin III: the intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked urine were between 85.0 and 101.1 %. The limit of detection was 3.0 and 2.3 ug/L, respectively. The preliminary reference ranges of coproporphyrin I and III in a group of donors are 5.0 51.6 ug/L (0.59 3.20 ug/mmol of creatinine) and 14.9 65.4 ug/L (0.98 8.21 ug/mmol of creatinine). The presented method is inexpensive and suitable for screening of porphyrias. (en)
Title
  • The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection
  • The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection (en)
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  • The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection
  • The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection (en)
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  • RIV/00216275:25310/10:39881874!RIV11-MSM-25310___
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  • 253737
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  • RIV/00216275:25310/10:39881874
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  • screening; porphyrias; high-performance liquid chromatography with fluorescence detection; coproporphyrin I and III (en)
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  • CZ - Česká republika
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  • [C3541E4C22DC]
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  • Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
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  • 16
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  • Kanďár, Roman
  • Štramová, Xenie
  • Žáková, Pavla
  • Bencová, Soňa
  • Frohlichová, Martina
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  • 1211-5541
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  • 25310
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