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Description
| - The determination of selected porphyrins, especially of coproporphyrin I and III is presently the most reliable direct approach to the screening of porphyrias. An HPLC method for the simultaneous measurement of coproporphyrin I and III in urine samples has been developed and evaluated. Separation of coproporphyrin I and III from interfering substances was achieved on reversed phase HPLC with gradient elution and fluorescence detection. Analytical performance of this method is satisfactory for both coproporphyrin I and coproporphyrin III: the intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked urine were between 85.0 and 101.1 %. The limit of detection was 3.0 and 2.3 ug/L, respectively. The preliminary reference ranges of coproporphyrin I and III in a group of donors are 5.0 51.6 ug/L (0.59 3.20 ug/mmol of creatinine) and 14.9 65.4 ug/L (0.98 8.21 ug/mmol of creatinine). The presented method is inexpensive and suitable for screening of porphyrias.
- The determination of selected porphyrins, especially of coproporphyrin I and III is presently the most reliable direct approach to the screening of porphyrias. An HPLC method for the simultaneous measurement of coproporphyrin I and III in urine samples has been developed and evaluated. Separation of coproporphyrin I and III from interfering substances was achieved on reversed phase HPLC with gradient elution and fluorescence detection. Analytical performance of this method is satisfactory for both coproporphyrin I and coproporphyrin III: the intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked urine were between 85.0 and 101.1 %. The limit of detection was 3.0 and 2.3 ug/L, respectively. The preliminary reference ranges of coproporphyrin I and III in a group of donors are 5.0 51.6 ug/L (0.59 3.20 ug/mmol of creatinine) and 14.9 65.4 ug/L (0.98 8.21 ug/mmol of creatinine). The presented method is inexpensive and suitable for screening of porphyrias. (en)
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Title
| - The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection
- The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection (en)
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skos:prefLabel
| - The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection
- The determination of coproporphyrin I and III in human urine using an HPLC with fluorescence detection (en)
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skos:notation
| - RIV/00216275:25310/10:39881874!RIV11-MSM-25310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/10:39881874
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - screening; porphyrias; high-performance liquid chromatography with fluorescence detection; coproporphyrin I and III (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Štramová, Xenie
- Žáková, Pavla
- Bencová, Soňa
- Frohlichová, Martina
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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