About: Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species

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About: Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An electrochemical biosensor for specific detection of DNA sequences from Aspergillus, a potentially aflatoxigenic fungus is reported. The detection technique was optimized using short DNA sequences, then applied for a 92-mer from the Aspergillus gene aflR that codes a biochemical pathway of aflatoxin B1 production. Voltammetric detection of the specific Aspergillus DNA sequence is based on hybridization of adsorbed target DNA with a biotinylated probe and subsequent binding with streptavidin alkaline phosphatase conjugate. Such modified electrode surface of carbon paste electrode (CPE) is incubated in a buffer with an electrochemically inactive substrate (1-naphthyl phosphate). Alkaline phosphatase converts 1-naphthyl phosphate into 1-naphthol, which is determined by the selected voltammetric technique. The optimized procedure provided good differentiation between complementary and noncomplementary probes and also between potentially aflatoxigenic and non aflatoxigenic fungi of the genus Aspergillus. An electrochemical biosensor for specific detection of DNA sequences from Aspergillus, a potentially aflatoxigenic fungus is reported. The detection technique was optimized using short DNA sequences, then applied for a 92-mer from the Aspergillus gene aflR that codes a biochemical pathway of aflatoxin B1 production. Voltammetric detection of the specific Aspergillus DNA sequence is based on hybridization of adsorbed target DNA with a biotinylated probe and subsequent binding with streptavidin alkaline phosphatase conjugate. Such modified electrode surface of carbon paste electrode (CPE) is incubated in a buffer with an electrochemically inactive substrate (1-naphthyl phosphate). Alkaline phosphatase converts 1-naphthyl phosphate into 1-naphthol, which is determined by the selected voltammetric technique. The optimized procedure provided good differentiation between complementary and noncomplementary probes and also between potentially aflatoxigenic and non aflatoxigenic fungi of the genus Aspergillus. (en)
Title	Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species (en)
skos:prefLabel	Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species (en)
skos:notation	RIV/00216275:25310/10:39881444!RIV13-GA0-25310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/09/0148), P(LC06035), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Metelka, Radovan Moťková, Petra Vytřasová, Jarmila Vytřas, Karel Brožková, Iveta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	249613
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/10:39881444
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	voltammetry; carbon paste electrode; DNA hybridization; Aspergillus fungi (en)
http://linked.open.../riv/klicoveSlovo	carbon paste electrode DNA hybridization voltammetry Aspergillus fungi
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[63DF7A1F9C3E]
http://linked.open...i/riv/nazevZdroje	Electrochemistry Communications
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Modern methods of detection and determination of microbial toxins Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Metelka, Radovan Vytřas, Karel Vytřasová, Jarmila Šnévajsová, Petra Brožková, Iveta Tisoň, Lukáš
http://linked.open...ain/vavai/riv/wos	000274233700026
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1388-2481
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.elecom.2009.10.047
http://localhost/t...ganizacniJednotka	25310

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