About: Mechanisms participating in oxidative damage of isolated rat hepatocytes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Study evaluated time course and dose dependence of peroxidative damage induced by tert-butyl hydroperoxide in rat hepatocytes cultured in suspension and in monolayer. At the lowest concentration, decrease of cytosolic glutathione and discharge of mitochondrial membrane potential could be detected. Significant increases in leakage of lactate dehydrogenase and in malondialdehyde concentrations together with decrease of pyruvate-dependent respiration were detected at higher tBHP concentrations and after longer incubation. Changes in plasma membrane integrity were observed at 1 mM concentration of tBHP. Succinate-dependent oxidation was most resistant to peroxidative damages. Opening of the mitochondrial permeability transition pore was responsible for the discharge of MMP. In the presence of cyclosporine A and succinate, the membrane potential could be restored. Data showed that the most sensitive indicators of the peroxidative damage are changes of cytosolic glutathione concentration and MMP. Study evaluated time course and dose dependence of peroxidative damage induced by tert-butyl hydroperoxide in rat hepatocytes cultured in suspension and in monolayer. At the lowest concentration, decrease of cytosolic glutathione and discharge of mitochondrial membrane potential could be detected. Significant increases in leakage of lactate dehydrogenase and in malondialdehyde concentrations together with decrease of pyruvate-dependent respiration were detected at higher tBHP concentrations and after longer incubation. Changes in plasma membrane integrity were observed at 1 mM concentration of tBHP. Succinate-dependent oxidation was most resistant to peroxidative damages. Opening of the mitochondrial permeability transition pore was responsible for the discharge of MMP. In the presence of cyclosporine A and succinate, the membrane potential could be restored. Data showed that the most sensitive indicators of the peroxidative damage are changes of cytosolic glutathione concentration and MMP. (en)
Title	Mechanisms participating in oxidative damage of isolated rat hepatocytes Mechanisms participating in oxidative damage of isolated rat hepatocytes (en)
skos:prefLabel	Mechanisms participating in oxidative damage of isolated rat hepatocytes Mechanisms participating in oxidative damage of isolated rat hepatocytes (en)
skos:notation	RIV/00216275:25310/09:00009093!RIV12-MSM-25310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA303/06/1261), Z(AV0Z50110509), Z(MSM0021620820), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Roušar, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	325198
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/09:00009093
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	tert-butyl hydroperoxide.; hepatocytes in culture; Oxidative stress (en)
http://linked.open.../riv/klicoveSlovo	Oxidative stress hepatocytes in culture tert-butyl hydroperoxide.
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F5EEA7FC0EC5]
http://linked.open...i/riv/nazevZdroje	Archives of Toxicology
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Pathophysiological function of mitochondrial glycerophopsphate dehydrogenase - production of reactive oxygen species
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	83
http://linked.open...iv/tvurceVysledku	Roušar, Tomáš Červinka, Miroslav Kučera, Otto Lotková, Halka Červinková, Zuzana Drahota, Zdeněk Endlicher, René Křiváková, Pavla Lábajová, Anna
http://linked.open...ain/vavai/riv/wos	000264883800009
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka The use of experimental and clinical models of metabolic processes, nutrition and pharmacotherapy for the advancement of knowledge, clinical practice and quality of life improvement Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	0340-5761
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00204-008-0385-8
http://localhost/t...ganizacniJednotka	25310

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