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  • An amperometric method for Salmonella detection is presented as an example of electroimmunoassay of bacterial cells. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10.00 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella Enteritidis in a sample
  • An amperometric method for Salmonella detection is presented as an example of electroimmunoassay of bacterial cells. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10.00 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella Enteritidis in a sample (en)
Title
  • Electroimmunoassay for detection of bacterial cells
  • Electroimmunoassay for detection of bacterial cells (en)
skos:prefLabel
  • Electroimmunoassay for detection of bacterial cells
  • Electroimmunoassay for detection of bacterial cells (en)
skos:notation
  • RIV/00216275:25310/09:00008382!RIV11-MSM-25310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06035), Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
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  • 312831
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/09:00008382
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • alkaline phosphatase; amperometric detection; carbon paste electrode; Salmonella Enteritidis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [32E855A815D1]
http://linked.open...i/riv/mistoVydani
  • Mountain View
http://linked.open...i/riv/nazevZdroje
  • Current Research topics in Applied Microbiology and Microbial Biotechnology
http://linked.open...in/vavai/riv/obor
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  • Vytřas, Karel
  • Vytřasová, Jarmila
  • Červenka, Libor
  • Pešková, Iva
  • Hrubeš, Michal
http://linked.open...n/vavai/riv/zamer
number of pages
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  • World Scientific Publishing Co.
https://schema.org/isbn
  • 978-981-283-754-7
http://localhost/t...ganizacniJednotka
  • 25310
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