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Description
| - The present study describes estimation of acetaminophen toxicity in cultured rat hepatocytes. We used different concentrations of AAP : 1, 2.5, 5, 10 and 20 mM, to test influence of AAP on cellular viability, functional capacity and oxidative status at given time intervals. WST-1 test showed decrease of dehydrogenase activity in 5, 10 and 20 mM AAP to 75% of control signal already after 1 hour of incubation. Functional capacity was tested by albumin assay where the decrease was strictly related to AAP dose. Intracellular oxidative status was assessed by analysis of GSH/GSSG levels and time course of ROS production and glutathione reductase (GR) activity. Increased ROS production was found already after 3 h of incubation in 2.5, 5, 10 and 20 mM AAP, respectively. The highest ROS production was measured after 12 h treatment. GR activity was decreased already after 3 h of incubation and remained also decreased in 2.5, 5, 10 and 20 mM AAP treated cells during further time of incubation.
- The present study describes estimation of acetaminophen toxicity in cultured rat hepatocytes. We used different concentrations of AAP : 1, 2.5, 5, 10 and 20 mM, to test influence of AAP on cellular viability, functional capacity and oxidative status at given time intervals. WST-1 test showed decrease of dehydrogenase activity in 5, 10 and 20 mM AAP to 75% of control signal already after 1 hour of incubation. Functional capacity was tested by albumin assay where the decrease was strictly related to AAP dose. Intracellular oxidative status was assessed by analysis of GSH/GSSG levels and time course of ROS production and glutathione reductase (GR) activity. Increased ROS production was found already after 3 h of incubation in 2.5, 5, 10 and 20 mM AAP, respectively. The highest ROS production was measured after 12 h treatment. GR activity was decreased already after 3 h of incubation and remained also decreased in 2.5, 5, 10 and 20 mM AAP treated cells during further time of incubation. (en)
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Title
| - Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro
- Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro (en)
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skos:prefLabel
| - Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro
- Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro (en)
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skos:notation
| - RIV/00216275:25310/09:00006531!RIV10-MSM-25310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/09:00006531
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Acetaminophen; oxidative stress; hepatocyte. (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Roušar, Tomáš
- Kučera, Otto
- Lotková, Halka
- Červinková, Zuzana
- Mužáková, Vladimíra
- Křiváková, Pavla
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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