About: Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro     Goto   Sponge   NotDistinct   Permalink

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  • The present study describes estimation of acetaminophen toxicity in cultured rat hepatocytes. We used different concentrations of AAP : 1, 2.5, 5, 10 and 20 mM, to test influence of AAP on cellular viability, functional capacity and oxidative status at given time intervals. WST-1 test showed decrease of dehydrogenase activity in 5, 10 and 20 mM AAP to 75% of control signal already after 1 hour of incubation. Functional capacity was tested by albumin assay where the decrease was strictly related to AAP dose. Intracellular oxidative status was assessed by analysis of GSH/GSSG levels and time course of ROS production and glutathione reductase (GR) activity. Increased ROS production was found already after 3 h of incubation in 2.5, 5, 10 and 20 mM AAP, respectively. The highest ROS production was measured after 12 h treatment. GR activity was decreased already after 3 h of incubation and remained also decreased in 2.5, 5, 10 and 20 mM AAP treated cells during further time of incubation.
  • The present study describes estimation of acetaminophen toxicity in cultured rat hepatocytes. We used different concentrations of AAP : 1, 2.5, 5, 10 and 20 mM, to test influence of AAP on cellular viability, functional capacity and oxidative status at given time intervals. WST-1 test showed decrease of dehydrogenase activity in 5, 10 and 20 mM AAP to 75% of control signal already after 1 hour of incubation. Functional capacity was tested by albumin assay where the decrease was strictly related to AAP dose. Intracellular oxidative status was assessed by analysis of GSH/GSSG levels and time course of ROS production and glutathione reductase (GR) activity. Increased ROS production was found already after 3 h of incubation in 2.5, 5, 10 and 20 mM AAP, respectively. The highest ROS production was measured after 12 h treatment. GR activity was decreased already after 3 h of incubation and remained also decreased in 2.5, 5, 10 and 20 mM AAP treated cells during further time of incubation. (en)
Title
  • Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro
  • Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro (en)
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  • Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro
  • Evaluation of Oxidative Status in Acetaminophen Treated Rat Hepatocytes Cultured in vitro (en)
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  • RIV/00216275:25310/09:00006531!RIV10-MSM-25310___
http://linked.open...avai/riv/aktivita
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  • Z(MSM0021627502)
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  • 2
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  • 313893
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  • RIV/00216275:25310/09:00006531
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  • Acetaminophen; oxidative stress; hepatocyte. (en)
http://linked.open.../riv/klicoveSlovo
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  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [8A08ADF29138]
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  • Physiological Reasearch
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  • 58
http://linked.open...iv/tvurceVysledku
  • Kanďár, Roman
  • Roušar, Tomáš
  • Kučera, Otto
  • Lotková, Halka
  • Červinková, Zuzana
  • Mužáková, Vladimíra
  • Křiváková, Pavla
http://linked.open...n/vavai/riv/zamer
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  • 0862-8408
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  • 25310
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