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rdf:type
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Description
| - Proteináza K byla imobilizovanána na magnetický nosič a ten byl aplikován do kanálku mikrofluidního zařízení. Mikročip byl použit ke štěpení fyziologického a patologického prionového proteinu. Další analýzou (Western blot) bylo potvrzeno, že obě formy lze snadno odlišit. (cs)
- A microreactor for proteinase K (PK)-mediated protein digestion was developed as a step towards the elaboration of a fully integrated microdevice for the detection of pathological prion protein (PrP). PK-grafted magnetic beads were immobilized inside a polydimethylsiloxane (PDMS) microchannel using a longitudinal magnetic field parallel to the flow direction and a magnetic field gradient, thereby forming a matrix for enzymatic digestion. This self-organization provided uniform pore sizes, a low flow resistance and a strong reaction efficiency due to a very thin diffusion layer. The microreactor?s performance was first evaluated using a model substrate, succinyl-ala-ala-ala-paranitroanilide (SAAAP). Reaction kinetics were typically accelerated a hundred-fold as compared to conventional batch reactions. Reproducibility was around 98% for on-chip experiments. This microsystem was then applied to the digestion of prion protein from brain tissues. Controlled proteolysis could be obtained by varying the on
- A microreactor for proteinase K (PK)-mediated protein digestion was developed as a step towards the elaboration of a fully integrated microdevice for the detection of pathological prion protein (PrP). PK-grafted magnetic beads were immobilized inside a polydimethylsiloxane (PDMS) microchannel using a longitudinal magnetic field parallel to the flow direction and a magnetic field gradient, thereby forming a matrix for enzymatic digestion. This self-organization provided uniform pore sizes, a low flow resistance and a strong reaction efficiency due to a very thin diffusion layer. The microreactor?s performance was first evaluated using a model substrate, succinyl-ala-ala-ala-paranitroanilide (SAAAP). Reaction kinetics were typically accelerated a hundred-fold as compared to conventional batch reactions. Reproducibility was around 98% for on-chip experiments. This microsystem was then applied to the digestion of prion protein from brain tissues. Controlled proteolysis could be obtained by varying the on (en)
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Title
| - Controlled proteolysis of normal and pathological prion protein in a microfluidic chip
- Controlled proteolysis of normal and pathological prion protein in a microfluidic chip (en)
- Kontrolovaná proteolýza fyziologického a patologického prionového proteinu v mikročipovém zařízení. (cs)
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skos:prefLabel
| - Controlled proteolysis of normal and pathological prion protein in a microfluidic chip
- Controlled proteolysis of normal and pathological prion protein in a microfluidic chip (en)
- Kontrolovaná proteolýza fyziologického a patologického prionového proteinu v mikročipovém zařízení. (cs)
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skos:notation
| - RIV/00216275:25310/08:00007542!RIV09-MSM-25310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/08:00007542
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Proteinase K; Prion protein; microfluidic device (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bílková, Zuzana
- Taverna, Myriam
- Viovy, Jean-Louis
- Slováková, Marcela
- Le Nell, Anne
- Smadja, Claire
- Minc, Nicolas
- Peyrin, Jean-Michel
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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