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| Description
| - Cíl. Vyvinout HPLC metodu pro diagnostiku porfyrií v rámci laboratorního cvičení. Metoda. Vyvinuli jsme metodu pro stanovení koproporfyrinů I a III v moči pomocí kapalinové chromatografie s fluorescenční detekcí. Pro odseparování vybraných porfyrinů od interferujících látek jsme použili gradientovou eluci. Průtok mobilní fáze byl udržován na hodnotě 0,5 ml/min. Koproporfyriny I a III byly detekovány při těchto vlnových délkách: excitace: 394 nm a emise 624 nm. Výsledky. Analytické parametry jsou pro klinické testy dostačující. Variační koeficienty přesnosti v sérii byly pro koproporfyrin je 4,84% a koproporfyrin III 6,00%. Kalibrační křivky byly v rozmezí 6-460 ug/l (koproporfyrin I) a 6-490 ug/l (koproporfyrin III) lineární. Výtěžnost metody byla pro koproporfyrin I 92,23% (CV 4,38%) a koproporfyrin III 99,05% (CV 5,41%). Předběžné referenční hodnoty u skupiny dárců byly v rozmezí 973 +/- 220 ug/mol kreatininu (koproporfyrin I) a 1842 +/- 888 ug/mol kreatininu (koproporfyrin III). Závěr. Tato meto (cs)
- Objective. To develop a HPLC method for the diagnosis of porphyrias within the laboratory training. Method. We have developed and evaluated a method for the determination of coproporphyrins I and III in urine by high-performance liquid chromatography with fluorescence detection. By using the controller, a gradient program was used to separate selected porphyrins from other interfering compounds. The flow rate was 0.5 mL/min. Coproporphyrins I and III were detected at excitation 394 nm and emission 624 nm. Results. The analytical performance of the method is satisfactory for clinical trials. The intra-assay coefficients of variation were 4.84% and 6.00% for coproporphyrin I and III, respectively. The calibration curve was linear with the tested range 6-460 ug/L for coproporphyrin I and 6-490 ug/L for coproporphyrin III. The recoveries were 92.23% (CV 4.38%) for coproporphyrin I and 99.05% (CV 5.41%) for coproporphyrin III. The preliminary reference range of coproporphyrins I and III in a group of don
- Objective. To develop a HPLC method for the diagnosis of porphyrias within the laboratory training. Method. We have developed and evaluated a method for the determination of coproporphyrins I and III in urine by high-performance liquid chromatography with fluorescence detection. By using the controller, a gradient program was used to separate selected porphyrins from other interfering compounds. The flow rate was 0.5 mL/min. Coproporphyrins I and III were detected at excitation 394 nm and emission 624 nm. Results. The analytical performance of the method is satisfactory for clinical trials. The intra-assay coefficients of variation were 4.84% and 6.00% for coproporphyrin I and III, respectively. The calibration curve was linear with the tested range 6-460 ug/L for coproporphyrin I and 6-490 ug/L for coproporphyrin III. The recoveries were 92.23% (CV 4.38%) for coproporphyrin I and 99.05% (CV 5.41%) for coproporphyrin III. The preliminary reference range of coproporphyrins I and III in a group of don (en)
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| Title
| - Determination of coproporphyrin I and coproporphyrin III in urine by HPLC with fluorescence detection
- Determination of coproporphyrin I and coproporphyrin III in urine by HPLC with fluorescence detection (en)
- Simultánní stanovení koproporfyrinu I a koproporfyrinu III v moči pomocí HPLC s fluorescenční detekcí (cs)
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| skos:prefLabel
| - Determination of coproporphyrin I and coproporphyrin III in urine by HPLC with fluorescence detection
- Determination of coproporphyrin I and coproporphyrin III in urine by HPLC with fluorescence detection (en)
- Simultánní stanovení koproporfyrinu I a koproporfyrinu III v moči pomocí HPLC s fluorescenční detekcí (cs)
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| skos:notation
| - RIV/00216275:25310/07:00006448!RIV08-MSM-25310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/07:00006448
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - porphyria; coproporphyrin I and III; liquid chromatography with fluorescence detection (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Klinická biochemie a metabolismus
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Skalický, Jiří
- Žáková, Pavla
- Mužáková, Vladimíra
- Frohlichová, Martina
- Kovařík, Jakub
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| issn
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Česká lékařská společnost J. E. Purkyně
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| http://localhost/t...ganizacniJednotka
| |
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