| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Background. Catecholamines are metabolized by monoamino oxidase and aldehyd dehydrogenase to vanillylmandelic acid (VMA) and homovanillic acid (HVA). The major metabolite of serotonin is 5-hydroxyindoleacetic acid (5-HIAA). A number of diseases are accompanied by increasing or decreased catecholamine metabolism. Methods. This method is based on selective isolation of VMA, HVA and 5-HIAA from urine samples on AG 1-X8 resin. Separation of VMA, HVA and 5-HIAA was achieved on reversed phase HPLC with isocratic elution and coulometric electrochemical detection (EG = 450 mV; E1 = 180 mV, gain range = 200 nA; E2 = 400 mV, gain range = 200 nA). Results. The analytical performance of this method is satisfactory with intra-assay CV 4.8% for VMA, CV 6.5% for HVA and 2.8% for 5-HIAA, inter-assay CV 8.2% for VMA, CV 10.2% for HVA and 8.4% for 5-HIAA, recovery 92.3% for VMA, 90.1% for HVA and 97.6% for 5-HIAA. The calibration curve was linear in the whole range tested (5.0 ? 500.0) umol/L. Conclusions. We have
- Background. Catecholamines are metabolized by monoamino oxidase and aldehyd dehydrogenase to vanillylmandelic acid (VMA) and homovanillic acid (HVA). The major metabolite of serotonin is 5-hydroxyindoleacetic acid (5-HIAA). A number of diseases are accompanied by increasing or decreased catecholamine metabolism. Methods. This method is based on selective isolation of VMA, HVA and 5-HIAA from urine samples on AG 1-X8 resin. Separation of VMA, HVA and 5-HIAA was achieved on reversed phase HPLC with isocratic elution and coulometric electrochemical detection (EG = 450 mV; E1 = 180 mV, gain range = 200 nA; E2 = 400 mV, gain range = 200 nA). Results. The analytical performance of this method is satisfactory with intra-assay CV 4.8% for VMA, CV 6.5% for HVA and 2.8% for 5-HIAA, inter-assay CV 8.2% for VMA, CV 10.2% for HVA and 8.4% for 5-HIAA, recovery 92.3% for VMA, 90.1% for HVA and 97.6% for 5-HIAA. The calibration curve was linear in the whole range tested (5.0 ? 500.0) umol/L. Conclusions. We have (en)
- Úvod. Katecholaminy jsou metabolizovány pomocí monoaminooxidasy a aldehyddehydrogenasy na kyselinu vanilmandlovou (VMK) a homovanilovou (HVK). Hlavním metabolitem odbourávání serotoninu je kyselina 5-hydroxyindoloctová (5-HIOK). Existuje celá řada onemocnění doprovázena buď zvýšenou nebo sníženou úrovní metabolismu katecholaminů. Metody. Metoda je založena na selektivní izolaci VMK, HVKa 5-HIOK z moče pomocí separace tuhou fází (AG 1-X8 pryskyřice). V dalším kroku jsou hladiny VMK, HVK a 5-HIOK v moči stanoveny pomocí RP-HPLC s coulometrickou elektrochemickou detekcí (EG = 450 mV; E1 = 180 mV, výstup 200 nA; E2 = 400 mV, výstup 200 nA). Výsledky. Analytické parametry prezentované metody jsou uspokojivé. Přesnost v sérii, vyjádřená variačním koeficientem, je pro VMK 4,8%, pro HVK 6,5% a pro 5-HIOK 2,8%. Přesnost mezi sériemi činí pro VMK 8,2%, HVK 10,2% a 8,4% pro 5-HIOK. Výtěžnost metody je pro VMK 92,3%, pro HVK 92,3% a pro 5-HIOK 97,6%. Kalibrační křivky byly pro výše uvedené látky lineární v rozs (cs)
|
| Title
| - Simultaneous determination of vanillylmandelic acid, homovanillic acid and 5-hydroxyindolacetic acid in urine by HPLC with electrochemical detection
- Simultánní stanovení kyseliny vanilmandlové, homovanilové a 5-hydroxyindoloctové v moči pomocí HPLC s elektrochemickou detekcí (cs)
- Simultaneous determination of vanillylmandelic acid, homovanillic acid and 5-hydroxyindolacetic acid in urine by HPLC with electrochemical detection (en)
|
| skos:prefLabel
| - Simultaneous determination of vanillylmandelic acid, homovanillic acid and 5-hydroxyindolacetic acid in urine by HPLC with electrochemical detection
- Simultánní stanovení kyseliny vanilmandlové, homovanilové a 5-hydroxyindoloctové v moči pomocí HPLC s elektrochemickou detekcí (cs)
- Simultaneous determination of vanillylmandelic acid, homovanillic acid and 5-hydroxyindolacetic acid in urine by HPLC with electrochemical detection (en)
|
| skos:notation
| - RIV/00216275:25310/07:00006443!RIV08-MSM-25310___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/07:00006443
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - vanillylmandelic acid; homovanillic acid; 5-hydroxyindolacetic acid; liquid chromatography with electrochemical detection (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| - Clinical Chemistry and Laboratory Medicine
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Skalický, Jiří
- Žáková, Pavla
- Kovařík, Jakub
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Walter de Gruyter GmbH & Co. KG
|
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)