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| rdf:type
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| Description
| - Vitamín D a jeho metabolity jsou důležité pro regulaci homeostázy vápníku a kostního metabolismu. Nejdůležitějšími metabolity vitamínu D jsou 25-hydroxyvitamín D3 a 1,25-dihydroxavitamín D3. Stanovení 25-hydroxyvitamínu D3 bylo provedeno pomocí vysoce účinné kapalinové chromatografie s UV detekcí. Pro separaci jsme zvolili jako stacionární fázi chromatografickou kolonu LiChroCart 125-4 Purospher Rp-18e, 5 um, mobilní fází byla směs methanolu a deionizované vody (95:5, v/v). Touto metodou jsme stanovovali hladiny 25-hydoxyvitamínu D3 u skupiny dobrovolných dárců krve. Předběžné hodnoty činí u této skupiny 10,42-116,20 nmol/l. Prováděli jsme statistickou analýzu porovnávající skupiny dárců se suplementací vitamínem D a bez suplementace. Žádné statisticky významné rozdíly jsme nenalezli. Vyvinuli jsme jednoduchou a selektivní HPLC metodu s UV detekcí pro stanovení 25-hydroxyvitamínu D3 v séru. Tato metoda poskytuje výbornou citlivost, přesnost a správnost a je vhodná pro výzkum, ale také pro klinické (cs)
- Vitamin D and its metabolites are important for the regulation of calcium homeostasis and for bone metabolism. The most important metabolites of vitamin D are 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. The determination of 25-hydroxyvitamin D3 was made by high-performance liquid chromatography with ultraviolet detection. For the separation, reverse phase column LiChroCart 125-4 Purospher RP-18e, 5 um, was used. The mixture of methanol and deionized water (95:5, v/v) was used as mobile phase. This method was applied to determination the concentration of 25-hydroxyvitamin D3 in a group of blood donors. The preliminary reference ranges of 25-hydroxyvitamin D3 in a group of blood donors are 10.42-116.20 nmol/L. The statistic analysis of the data measured was processed, including comparing the values obtained from the blood donors with supplementation of vitamin D and the ones without the supplementation of vitamin D. No statistically significant data diversity was found out in the analysis. We
- Vitamin D and its metabolites are important for the regulation of calcium homeostasis and for bone metabolism. The most important metabolites of vitamin D are 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3. The determination of 25-hydroxyvitamin D3 was made by high-performance liquid chromatography with ultraviolet detection. For the separation, reverse phase column LiChroCart 125-4 Purospher RP-18e, 5 um, was used. The mixture of methanol and deionized water (95:5, v/v) was used as mobile phase. This method was applied to determination the concentration of 25-hydroxyvitamin D3 in a group of blood donors. The preliminary reference ranges of 25-hydroxyvitamin D3 in a group of blood donors are 10.42-116.20 nmol/L. The statistic analysis of the data measured was processed, including comparing the values obtained from the blood donors with supplementation of vitamin D and the ones without the supplementation of vitamin D. No statistically significant data diversity was found out in the analysis. We (en)
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| Title
| - HPLC assay for 25-hydroxyvitamin D3 in serum
- HPLC assay for 25-hydroxyvitamin D3 in serum (en)
- HPLC stanovení 25-hydroxyvitamínu D3 v séru (cs)
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| skos:prefLabel
| - HPLC assay for 25-hydroxyvitamin D3 in serum
- HPLC assay for 25-hydroxyvitamin D3 in serum (en)
- HPLC stanovení 25-hydroxyvitamínu D3 v séru (cs)
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| skos:notation
| - RIV/00216275:25310/07:00006367!RIV08-MSM-25310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/07:00006367
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - vitamin D; 25-hydroxyvitamin D3; serum; liquid chromatography with ultraviolet detection (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Vitamins 2007 - Nutrition and Diagnostics
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Skalický, Jiří
- Žáková, Pavla
- Kovařík, Jakub
- Navrátilová, Barbora
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
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