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| rdf:type
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| Description
| - Beta-karoten hraje v biologických systémech významnou úlohu. Chrání organismus proti účinku reaktivních sloučenin kyslíku a dusíku. Vyvinuli jsme metodu pro stanovení beta-karotenu v lidské plazmě. Pro separaci jsme vybrali jako stacionární fázi chromatografickou kolonu LiChroCart 125-4 Purospher RP-18e, 5 um, mobilní fází byla směs methanolu a ethanolu (75:25, v/v). Jako vnitřní standard jsme používali retinylpalmitát. Analytické parametry předkládané metody jsou uspokojivé, přesnost v sérii byla s variačním koeficientem pod 10% a výtěžnost metody se pohybovala v rozmezí 90-100%. Kalibrační křivka byla lineární v celém rozmezí (0,02-2,00) umol/l. Detekční limit byl 0,5 pmol/20 ul nástřik. Tato metoda je reprodukovatelná a zcela vhodná pro stanovení beta-karotenu v lidské plazmě. Poděkování. Tato práce byla podpořena grantem MSM 0021627502. (cs)
- Beta-carotene plays an important role in biological systems. It protects against the action of some reactive oxygen and nitrogen species. A method for determination of beta-carotene in human plasma has been developed and evaluated. Chromatography was accomplished using isocratic elution on LiChrocart 125-4 Purospher RP-18e, 5 um, column. The mixture of methanol and ethanol (75:25, v/v) was used as a mobile phase. As internal standard was chosen retinyl palmitate. The analytical performance of this method is satisfactory, both intra-assay and inter-assay were with coefficients of variation below 10%, recovery was between 90-110%. The calibration curve was linear in the whole range tested (0.02-2.00) umol/L. The detection limit was 0.5 pmol/20 ul inject. This assay is a suitable and reproducible HPLC method for the determination of beta-carotene in human plasma. Acknowledgments. This work was supported by grant MSM0021627502.
- Beta-carotene plays an important role in biological systems. It protects against the action of some reactive oxygen and nitrogen species. A method for determination of beta-carotene in human plasma has been developed and evaluated. Chromatography was accomplished using isocratic elution on LiChrocart 125-4 Purospher RP-18e, 5 um, column. The mixture of methanol and ethanol (75:25, v/v) was used as a mobile phase. As internal standard was chosen retinyl palmitate. The analytical performance of this method is satisfactory, both intra-assay and inter-assay were with coefficients of variation below 10%, recovery was between 90-110%. The calibration curve was linear in the whole range tested (0.02-2.00) umol/L. The detection limit was 0.5 pmol/20 ul inject. This assay is a suitable and reproducible HPLC method for the determination of beta-carotene in human plasma. Acknowledgments. This work was supported by grant MSM0021627502. (en)
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| Title
| - Determination of beta-carotene in human plasma by HPLC with ultraviolet detection
- Stanovení beta-karotenu v lidské plazmě pomocí HPLC s UV detekcí (cs)
- Determination of beta-carotene in human plasma by HPLC with ultraviolet detection (en)
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| skos:prefLabel
| - Determination of beta-carotene in human plasma by HPLC with ultraviolet detection
- Stanovení beta-karotenu v lidské plazmě pomocí HPLC s UV detekcí (cs)
- Determination of beta-carotene in human plasma by HPLC with ultraviolet detection (en)
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| skos:notation
| - RIV/00216275:25310/07:00006169!RIV08-MSM-25310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/07:00006169
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - beta-carotene; liquid chromatography with ultraviolet detection; retinyl palmitate (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Vitamins 2007 - Nutrition and Diagnostics
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Kanďár, Roman
- Žáková, Pavla
- Mužáková, Vladimíra
- Kulichová, Bohumila
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| http://linked.open...n/vavai/riv/zamer
| |
| number of pages
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| http://purl.org/ne...btex#hasPublisher
| |
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
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