About: Detection of toxigenic fusarium from foods by PCR

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR (cs) Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 bp. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphological features are important for species classification where the PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. Thus the PCR technique has proved itself a reli Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 bp. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphological features are important for species classification where the PCR can distinguish between toxigenic and non-toxigenic fungi of Fusarium. Thus the PCR technique has proved itself a reli (en)
Title	Detection of toxigenic fusarium from foods by PCR Detection of toxigenic fusarium from foods by PCR (en) Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR (cs)
skos:prefLabel	Detection of toxigenic fusarium from foods by PCR Detection of toxigenic fusarium from foods by PCR (en) Detekce toxinogenních plísní rodu Fusarium v potravinách metodou PCR (cs)
skos:notation	RIV/00216275:25310/07:00005992!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany	73-85
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2106), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Červenka, Libor Pejchalová, Marcela Moťková, Petra Vytřasová, Jarmila Brožková, Iveta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	416556
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/07:00005992
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Fusarium spp.; Fusarium graminearu; , toxigenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins (en)
http://linked.open.../riv/klicoveSlovo	polymerase chain reaction trichothecenes mycotoxins Fusarium spp. Fusarium graminearu toxigenic fungi Tri5 gene
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[FF7003C3EE97]
http://linked.open...i/riv/nazevZdroje	Scientific Papers of the University of Pardubice, Series A
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Modern instrumentation in chemical, food and clinical analysis
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Pejchalová, Marcela Vytřasová, Jarmila Šnévajsová, Petra Červenka, Libor Svobodová, Lucie Brožková, Iveta
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1211-5541
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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