About: Comparison of aflatoxinogenic fungal DNA isolation for PCR.

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	určeno pro seznam publikací (cs) Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol?ES. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration. The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium. Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol?ES. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration. The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium. (en)
Title	Comparison of aflatoxinogenic fungal DNA isolation for PCR. Comparison of aflatoxinogenic fungal DNA isolation for PCR. (en) Porovnání jednotlivých metod izolace DNA z aflatoxinogenních plísní. (cs)
skos:prefLabel	Comparison of aflatoxinogenic fungal DNA isolation for PCR. Comparison of aflatoxinogenic fungal DNA isolation for PCR. (en) Porovnání jednotlivých metod izolace DNA z aflatoxinogenních plísní. (cs)
skos:notation	RIV/00216275:25310/06:00004295!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany	453
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2106), Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Pejchalová, Marcela Moťková, Petra Vytřasová, Jarmila Brožková, Iveta
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	469221
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/06:00004295
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	aflatoxigenic fungi; DNA isolation; PCR (en)
http://linked.open.../riv/klicoveSlovo	DNA isolation PCR aflatoxigenic fungi
http://linked.open...ontrolniKodProRIV	[A72DBC7470BB]
http://linked.open...i/riv/mistoVydani	Bologna
http://linked.open...i/riv/nazevZdroje	The Proceedings: FoodMicro 2006, Food safety and food biotechnology: diversity and global impact
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Modern instrumentation in chemical, food and clinical analysis
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Pejchalová, Marcela Vytřasová, Jarmila Šnévajsová, Petra Brožková, Iveta
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Alma Mater Studiorum, University of Bologna
https://schema.org/isbn	961-90346-3-5
http://localhost/t...ganizacniJednotka	25310

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