About: Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR.

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About: Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sixty nine meat and water samples were examined by m?PCR assay of the enrichment media. Twenty nine (42 %) of samples were contaminated with Arcobacter species. In 17 samples (24,6 %), A. butzleri was confirmed by m?PCR. Direct detection of arcobacters from assay avoided cultivation on agar plates and reduced the time required for detection. Sensitivity of optimised m-PCR method was verified using four different enrichment broths artificially contaminated with pure cultures of Arcobacters. Formula 6 gave the best results from recommended enrichment media. Influence of matrix samples was tested on real samples of chicken, pork and beef, in which suspension of definite density of Arcobacters was added. It was shown, particularly, that a sample with higher fat content has required a longer incubation period in enrichment broth for successful detection by m?PCR assay. Sixty nine meat and water samples were examined by m?PCR assay of the enrichment media. Twenty nine (42 %) of samples were contaminated with Arcobacter species. In 17 samples (24,6 %), A. butzleri was confirmed by m?PCR. Direct detection of arcobacters from assay avoided cultivation on agar plates and reduced the time required for detection. Sensitivity of optimised m-PCR method was verified using four different enrichment broths artificially contaminated with pure cultures of Arcobacters. Formula 6 gave the best results from recommended enrichment media. Influence of matrix samples was tested on real samples of chicken, pork and beef, in which suspension of definite density of Arcobacters was added. It was shown, particularly, that a sample with higher fat content has required a longer incubation period in enrichment broth for successful detection by m?PCR assay. (en) Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR. (cs)
Title	Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR. Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR. (en) Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR. (cs)
skos:prefLabel	Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR. Occurrence of Arcobacters in Czech Republic and the influence of sample matrix on their detection using PCR. (en) Výskyt arkobakterů v ČR a vliv matrice vzorku na jejich detekci metodou PCR. (cs)
skos:notation	RIV/00216275:25310/06:00004122!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany	152-158
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2106), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Červenka, Libor Pejchalová, Marcela Vytřasová, Jarmila Brožková, Iveta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	489808
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/06:00004122
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Arcobacter cryaerophilus; Arcobacter butzleri; multiplex-PCR; matrix of samples (en)
http://linked.open.../riv/klicoveSlovo	Arcobacter cryaerophilus Arcobacter butzleri matrix of samples multiplex-PCR
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[A414D9B9DE4C]
http://linked.open...i/riv/nazevZdroje	Journal of Food and Nutrition Research
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Modern instrumentation in chemical, food and clinical analysis
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	45
http://linked.open...iv/tvurceVysledku	Husková, Zuzana Pejchalová, Marcela Vytřasová, Jarmila Červenka, Libor Brožková, Iveta
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1336-8672
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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