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| rdf:type
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| Description
| - Protože S-adenosylmethionin (SAMe) má možný hepatoprotektivní efekt, zjišťovali jsme schopnost SAMe zlepšit funkci hepatocytů potkanů poškozených D-galaktosaminem (GalN). Izolované hepatocyty potkanů byly umístěny v mediu a byla zjištěna jejich funkční kapacita a integrita (via měření sekrece albuminu, syntesa urey, LDH aktivity a aktivita mitochondriálních dehydrogenas). Z výsledků vyplynulo, že SAMe snižuje toxicitu indukovanou GalN na dávce dependentním způsobem. (cs)
- Being a key intermediate in transmethylation and transsulphuration reactions, S-adenosyl-L-methionine (SAMe) exerts a possible hepatoprotective effect. We evaluated the ability of SAMe to improve the functions of rat hepatocytes damaged by D-galactosamine (GalN). Hepatocytes were isolated from Wistar rats (220 ? 250 g) by collagenase perfusion and cultured in William´s E medium. After cell attachment GalN (40 mM) +/- SAMe (50 and 1000 mg/L) were added to media. Hepatocyte functional capacity and integrity were analysed by albumin secretion activity (ELISA kit, Bethyl Lab.), urea synthesis (kit, Sigma Aldrich) and lactate dehydrogenase (LDH) activity (kit, Sigma Aldrich) in cultivation media after 24 and 48 h incubation. Activities of mitochondrial dehydrogenases were determined by colorimetric assay based on the cleavage of the tetrazolium salt WST?1. GalN increased significantly LDH leakage from the cells during 24 h and 48 h incubation and decreased albumin secretion activity. SAMe significantly red
- Being a key intermediate in transmethylation and transsulphuration reactions, S-adenosyl-L-methionine (SAMe) exerts a possible hepatoprotective effect. We evaluated the ability of SAMe to improve the functions of rat hepatocytes damaged by D-galactosamine (GalN). Hepatocytes were isolated from Wistar rats (220 ? 250 g) by collagenase perfusion and cultured in William´s E medium. After cell attachment GalN (40 mM) +/- SAMe (50 and 1000 mg/L) were added to media. Hepatocyte functional capacity and integrity were analysed by albumin secretion activity (ELISA kit, Bethyl Lab.), urea synthesis (kit, Sigma Aldrich) and lactate dehydrogenase (LDH) activity (kit, Sigma Aldrich) in cultivation media after 24 and 48 h incubation. Activities of mitochondrial dehydrogenases were determined by colorimetric assay based on the cleavage of the tetrazolium salt WST?1. GalN increased significantly LDH leakage from the cells during 24 h and 48 h incubation and decreased albumin secretion activity. SAMe significantly red (en)
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| Title
| - The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine.
- The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine. (en)
- Vliv S-adenosylmethioninu na funkci hepatocytů potkanů poškozených D-galaktosaminem (cs)
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| skos:prefLabel
| - The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine.
- The in vitro effect of S-adenosyl-L-methionine on the function of rat hepatocytes damaged by D-galactosamine. (en)
- Vliv S-adenosylmethioninu na funkci hepatocytů potkanů poškozených D-galaktosaminem (cs)
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| skos:notation
| - RIV/00216275:25310/05:00002958!RIV08-MSM-25310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/05:00002958
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - S-adenosyl-L-methionine; rat hepatocytes; D-galactosamine. (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Roušar, Tomáš
- Kučera, Otto
- Lotková, Halka
- Červinková, Zuzana
- Héžová, Renata
- Mužáková, Vladimíra
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
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| http://localhost/t...ganizacniJednotka
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