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| rdf:type
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| Description
| - Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. ATCH is used for testing of enzymatic hydrolysis of ACH in vitro, because mechanism of ATCH hydrolysis is qualitatively similar to ACH and its reaction course can be quantitatively on-line measured by two independent methods: spectrophotometrical (determination of thiocholine - product of ATCH hydrolysis - using Ellman´s method) and electrochemical (determination of acetic acid - product of ATCH hydrolysis - by pH-stat method). All tested hydrolyses correspond to the Michaelis-Menten´s equation with the second irreversible step up to the total exhaustion of the substrate. The correlations were made by means of differential and integral kinetic equations describing Michaelis-Menten model. The optimal values of Michaelis constant (KM), maximum velocity (Vm), kinetic constants of single reaction steps and absolute concentration of the used enzyme were
- Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. ATCH is used for testing of enzymatic hydrolysis of ACH in vitro, because mechanism of ATCH hydrolysis is qualitatively similar to ACH and its reaction course can be quantitatively on-line measured by two independent methods: spectrophotometrical (determination of thiocholine - product of ATCH hydrolysis - using Ellman´s method) and electrochemical (determination of acetic acid - product of ATCH hydrolysis - by pH-stat method). All tested hydrolyses correspond to the Michaelis-Menten´s equation with the second irreversible step up to the total exhaustion of the substrate. The correlations were made by means of differential and integral kinetic equations describing Michaelis-Menten model. The optimal values of Michaelis constant (KM), maximum velocity (Vm), kinetic constants of single reaction steps and absolute concentration of the used enzyme were (en)
- Byla sledována kinetika enzymatické hydrolýzy acetylthiocholinu a acetylcholinu cholinesterázami. Všechny testované hydrolýzy splňují až do totálního vyčerpání substrátu model Michaelis-Mentenové s druhým irreversibilním krokem. Na základě experimentálních dat byly vypočteny optimální hodnoty Michaelisovy konstanty KM, maximální rychlost Vm a kinetické konstanty jednotlivých reakčních kroků pro všechny testované systémy (cs)
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| Title
| - Kinetics of Hydrolysis of Acetylthiocholine and Acetylcholine by Cholinesterases.
- Kinetics of Hydrolysis of Acetylthiocholine and Acetylcholine by Cholinesterases. (en)
- Kinetika hydrolýzy acetylthiocholinu a acetylcholinu pomocí cholinesteráz (cs)
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| skos:prefLabel
| - Kinetics of Hydrolysis of Acetylthiocholine and Acetylcholine by Cholinesterases.
- Kinetics of Hydrolysis of Acetylthiocholine and Acetylcholine by Cholinesterases. (en)
- Kinetika hydrolýzy acetylthiocholinu a acetylcholinu pomocí cholinesteráz (cs)
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| skos:notation
| - RIV/00216275:25310/05:00002848!RIV08-MSM-25310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/05:00002848
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - enzymatic hydrolysis; acetylcholine; acetylthiocholine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Chemico-Biological Interactions
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Komers, Karel
- Komersová, Alena
- Zdražilová, Pavla
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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