About: Occurence of Fusarium in food and feed

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	určeno pro seznam publikací (cs) Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphologic Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food and feedstuff samples were examined for the presence of trichothecenes-producing Fusarium. In total, seven Fusarium species were isolated, of which five reacted positively with the primer, resulting in the expected size amplicon of 260 pb. Macroscopic and microscopic features were observed on the pure culture of Fusarium and on fungi isolated in relation to their growth in different laboratory media. Morphologic (en)
Title	Occurence of Fusarium in food and feed Occurence of Fusarium in food and feed (en) Plísně rodu Fusarium v potravinách a krmivech (cs)
skos:prefLabel	Occurence of Fusarium in food and feed Occurence of Fusarium in food and feed (en) Plísně rodu Fusarium v potravinách a krmivech (cs)
skos:notation	RIV/00216275:25310/05:00002650!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany	37-38
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2106), Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Červenka, Libor Vytřasová, Jarmila Brožková, Iveta
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	533927
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/05:00002650
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Fusarium spp.; Fusarium graminearum; toxinogenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins (en)
http://linked.open.../riv/klicoveSlovo	polymerase chain reaction Fusarium graminearum trichothecenes mycotoxins Fusarium spp. toxinogenic fungi Tri5 gene
http://linked.open...ontrolniKodProRIV	[46B173C38013]
http://linked.open...v/mistoKonaniAkce	Olomouc
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Czech Mycology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Modern instrumentation in chemical, food and clinical analysis
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Vytřasová, Jarmila Červenka, Libor Svobodová, Lucie Brožková, Iveta
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2005-09-02 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1211-0981
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Česká vědecká společnost pro mykologii
http://localhost/t...ganizacniJednotka	25310

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