About: TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC     Goto   Sponge   NotDistinct   Permalink

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Description
  • OBJECTIVES: Lung cancer with the ALK rearrangement constitutes only a small fraction of patients with non-small cell lung cancer (NSCLC). However, in the era of molecular-targeted therapy, efficient patient selection is crucial for successful treatment. In this context, an effective method for EML4-ALK detection is necessary. We developed a new highly sensitive variant specific TaqMan based real time PCR assay applicable to RNA from formalin-fixed paraffin-embedded tissue (FFPE). MATERIALS AND METHODS: This assay was used to analyze the EML4-ALK gene in 96 non-selected NSCLC specimens and compared with two other methods (end-point PCR and break-apart FISH). RESULTS: EML4-ALK was detected in 33/96 (34%) specimens using variant specific real time PCR, whereas in only 23/96 (24%) using end-point PCR. All real time PCR positive samples were confirmed with direct sequencing. A total of 46 specimens were subsequently analyzed by all three detection methods.
  • OBJECTIVES: Lung cancer with the ALK rearrangement constitutes only a small fraction of patients with non-small cell lung cancer (NSCLC). However, in the era of molecular-targeted therapy, efficient patient selection is crucial for successful treatment. In this context, an effective method for EML4-ALK detection is necessary. We developed a new highly sensitive variant specific TaqMan based real time PCR assay applicable to RNA from formalin-fixed paraffin-embedded tissue (FFPE). MATERIALS AND METHODS: This assay was used to analyze the EML4-ALK gene in 96 non-selected NSCLC specimens and compared with two other methods (end-point PCR and break-apart FISH). RESULTS: EML4-ALK was detected in 33/96 (34%) specimens using variant specific real time PCR, whereas in only 23/96 (24%) using end-point PCR. All real time PCR positive samples were confirmed with direct sequencing. A total of 46 specimens were subsequently analyzed by all three detection methods. (en)
Title
  • TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC
  • TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC (en)
skos:prefLabel
  • TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC
  • TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC (en)
skos:notation
  • RIV/00216224:14740/14:00077719!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED1.1.00/02.0068), P(EE2.3.20.0045)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 49307
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14740/14:00077719
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Lung cancer; Targeted therapy; NSCLC; EML4-ALK; Real time PCR; FISH (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • IE - Irsko
http://linked.open...ontrolniKodProRIV
  • [159502C0DDD7]
http://linked.open...i/riv/nazevZdroje
  • Lung Cancer
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 85
http://linked.open...iv/tvurceVysledku
  • Dvořáková, Dana
  • Mayer, Jiří
  • Pospíšilová, Šárka
  • Skřičková, Jana
  • Tomíšková, Marcela
  • Bajerová, Monika
  • Robešová, Blanka
  • Lišková, Květoslava
http://linked.open...ain/vavai/riv/wos
  • 000338617100005
issn
  • 0169-5002
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.lungcan.2014.04.002
http://localhost/t...ganizacniJednotka
  • 14740
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