About: Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?     Goto   Sponge   NotDistinct   Permalink

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  • In eukaryotic phyla studied so far, the essential centromeric histone H3 variant (CENH3) is loaded to centromeric nucleosomes after S-phase (except for yeast) but before mitotic segregation (except for metazoan). While the C-terminal part of CENH3 seems to be sufficient for mitotic centromere function in plants, meiotic centromeres neither load nor tolerate impaired CENH3 molecules. However, details about CENH3 deposition in meiocytes are unknown (except for Drosophila). Therefore, we quantified fluorescence signals after the immunostaining of CENH3 along meiotic and mitotic nuclear division cycles of rye, a monocotyledonous plant. One peak of fluorescence intensity appeared in the early meiotic prophase of pollen mother cells and a second one during interkinesis, both followed by a decrease of CENH3. Then, the next loading occurred in the male gametophyte before its first mitotic division. These data indicate that CENH3 loading differs between mitotic and meiotic nuclei.
  • In eukaryotic phyla studied so far, the essential centromeric histone H3 variant (CENH3) is loaded to centromeric nucleosomes after S-phase (except for yeast) but before mitotic segregation (except for metazoan). While the C-terminal part of CENH3 seems to be sufficient for mitotic centromere function in plants, meiotic centromeres neither load nor tolerate impaired CENH3 molecules. However, details about CENH3 deposition in meiocytes are unknown (except for Drosophila). Therefore, we quantified fluorescence signals after the immunostaining of CENH3 along meiotic and mitotic nuclear division cycles of rye, a monocotyledonous plant. One peak of fluorescence intensity appeared in the early meiotic prophase of pollen mother cells and a second one during interkinesis, both followed by a decrease of CENH3. Then, the next loading occurred in the male gametophyte before its first mitotic division. These data indicate that CENH3 loading differs between mitotic and meiotic nuclei. (en)
Title
  • Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?
  • Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis? (en)
skos:prefLabel
  • Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?
  • Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis? (en)
skos:notation
  • RIV/00216224:14740/14:00077480!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita
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  • P(EE2.3.20.0189)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
  • Schubert, Ingo
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  • 26428
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  • RIV/00216224:14740/14:00077480
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  • CELL-CYCLE PROGRESSION; CENP-A; CHROMOSOME; DYNAMICS; HETEROCHROMATIN; SEGREGATION; REQUIRES; PLANTS (en)
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  • US - Spojené státy americké
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  • [AD673409B37C]
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  • Chromosoma
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  • 123
http://linked.open...iv/tvurceVysledku
  • Schubert, Ingo
  • Lermontova, Inna
  • Schubert, Vei
http://linked.open...ain/vavai/riv/wos
  • 000342465800008
issn
  • 0009-5915
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s00412-014-0466-9
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  • 14740
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