About: Enzyme-linked electrochemical DNA ligation assay using magnetic beads

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007%2Fs00216-014-7811-y
Description	DNA ligases are essential enzymes in all cells and have been proposed as targets for novel antibiotics. Efficient DNA ligase activity assays are thus required for applications in biomedical research. Here we present an enzyme-linked electrochemical assay based on two terminally tagged probes forming a nicked junction upon hybridization with a template DNA. Nicked DNA bearing a 5 biotin tag is immobilized on the surface of streptavidin-coated magnetic beads, and ligated product is detected via a 3 digoxigenin tag recognized by monoclonal antibody-alkaline phosphatase conjugate. Enzymatic conversion of napht-1-yl phosphate to napht-1-ol enables sensitive detection of the voltammetric signal on a pyrolytic graphite electrode. DNA ligases are essential enzymes in all cells and have been proposed as targets for novel antibiotics. Efficient DNA ligase activity assays are thus required for applications in biomedical research. Here we present an enzyme-linked electrochemical assay based on two terminally tagged probes forming a nicked junction upon hybridization with a template DNA. Nicked DNA bearing a 5 biotin tag is immobilized on the surface of streptavidin-coated magnetic beads, and ligated product is detected via a 3 digoxigenin tag recognized by monoclonal antibody-alkaline phosphatase conjugate. Enzymatic conversion of napht-1-yl phosphate to napht-1-ol enables sensitive detection of the voltammetric signal on a pyrolytic graphite electrode. (en)
Title	Enzyme-linked electrochemical DNA ligation assay using magnetic beads Enzyme-linked electrochemical DNA ligation assay using magnetic beads (en)
skos:prefLabel	Enzyme-linked electrochemical DNA ligation assay using magnetic beads Enzyme-linked electrochemical DNA ligation assay using magnetic beads (en)
skos:notation	RIV/00216224:14740/14:00075856!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP206/11/1638), P(GPP206/11/P739), P(IAA400040901)
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Fojta, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	14820
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00075856
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Electrochemistry; Enzyme labeling; DNA ligase; Nicked DNA; Magnetic beads; Immobilized assays (en)
http://linked.open.../riv/klicoveSlovo	DNA ligase Enzyme labeling Immobilized assays Magnetic beads Nicked DNA Electrochemistry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[CDBE778CE7FC]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel electrochemical sensors and sensing techniques for the analysis of nucleic acids structure and interactions Sequence-specific electrochemical sensing of PCR-amplified genomic DNA fragments DNA labeling with redox markers for electrochemical sensing. Applications in analysis of nucleotide sequences and molecular diagnostics
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	406
http://linked.open...iv/tvurceVysledku	Fojta, Miroslav Horáková, Petra Stejskalová, Eva Vacek, Jan Bowater, Richard
http://linked.open...ain/vavai/riv/wos	000337787400010
issn	1618-2642
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00216-014-7811-y
http://localhost/t...ganizacniJednotka	14740

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