About: Automated Microscopy in Forward Genetic Screening of Arabidopsis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_4
Description	Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest. Tightly controlled spatiotemporal specificity of gene expression is intrinsic to developmental and adaptation responses of living systems throughout the kingdoms. Forward genetic screens employing well-characterized reporter lines can be used to identify as yet unknown genetic factors driving specific aspects of individual regulatory pathways. However, such screens are demanding with respect to data acquisition and analysis from thousands of mutant lines. Here, we describe a method that allows screening of a mutagenized GUS reporter line in Arabidopsis using an automated microscopy imaging system as a tool for rapid and efficient identification of mutants with modified expression profile for a gene of interest. (en)
Title	Automated Microscopy in Forward Genetic Screening of Arabidopsis Automated Microscopy in Forward Genetic Screening of Arabidopsis (en)
skos:prefLabel	Automated Microscopy in Forward Genetic Screening of Arabidopsis Automated Microscopy in Forward Genetic Screening of Arabidopsis (en)
skos:notation	RIV/00216224:14740/14:00074474!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(EE2.3.20.0189), P(GA13-25280S), P(GAP501/11/1150)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	4565
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00074474
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	apical meristem; Arabidopsis thaliana; branching morphogenesis; cell communication; cell function; cell membrane; cell shape (en)
http://linked.open.../riv/klicoveSlovo	cell membrane Arabidopsis thaliana cell communication apical meristem branching morphogenesis cell function cell shape
http://linked.open...ontrolniKodProRIV	[40F1C98E36EE]
http://linked.open...v/mistoKonaniAkce	New York
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...i/riv/nazevZdroje	Plant Cell Morphogenesis: Methods and Protocols
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of excellence in evolutionary cytogenomics, epigenetics and cell signalling Objasnění molekulárních mechanizmů interakce cytokininů a etylénu ve vývoji rostlin The role of cytokinins and auxin interactions in the regulation of root gravitropism in Arabidopsis thaliana. Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Hejátko, Jan Dobisová, Tereza
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2014-01-01 (xsd:date)
issn	1064-3745
number of pages	14 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-643-6_4
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	9781627036429
http://localhost/t...ganizacniJednotka	14740

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